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Rb X Ms Angiopoietin-2

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货号：AB3121

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M ELISA, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3121
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Angiopoietin-2 Antibody, NT
Alternate Names
  • Ang-2
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5
Applications
Application Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen 20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.
Epitope N-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.
Species Reactivity
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
Gene Symbol
  • ANGPT2
  • ANG-2
  • Tie2-ligand
  • ANG2
  • angiopoietin-2a
  • AGPT2
  • Ang2
  • angiopoietin-2B
  • angiopoietin-2
UniProt Number
UniProt Summary FUNCTION: SwissProt: O15123 # Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
SIZE: 496 amino acids; 56919 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: O15123 ## Contains 1 fibrinogen C-terminal domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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