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Rb X CDC2 phospho Tyr15

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货号：AB3241-25UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3241-25UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Cdc2 Antibody, phospho-specific (Tyr15)
Alternate Names
  • Cell Division Cycle 2
  • p34 Protein Kinase
  • Cyclin-dependent Kinase 1
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Applications
Application Use Anti-Cdc2 Antibody, phospho-specific (Tyr15) (Rabbit Polyclonal Antibody) validated in WB to detect Cdc2 also known as Cell Division Cycle 2, p34 Protein Kinase.
Key Applications
  • Western Blotting
Application Notes Western blot: 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide corresponding to amino acids surrounding the phosphoTry15 of human, rat, mouse and Xenopus CDC2.
Epitope phospho-specific (Tyr15)
Host Rabbit
Specificity CDC2, phosphoTyr15. By Western blot the antibody recognizes the ~35 kDa phospho CDC2 protein. The labeling of the CDC2 band in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Gene Symbol
  • CDC2
  • CDK1
  • DKFZp686L20222
  • MGC111195
  • CDC28A
  • EC 2.7.11.22
  • EC 2.7.11.23
Modifications
  • Phosphorylation
UniProt Number
UniProt Summary FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Packaging Information
Material Size 25 µL
Transport Information
Supplemental Information
Specifications

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