Rb X Endothelin Receptor A

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	ICC, IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ICC, IHC, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB3260-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT
Alternate Names	ET-A

Description

Catalogue Number

AB3260-200UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, CT

Alternate Names

ET-A

Product Information
Format	Affinity Purified
Control	CONTROL ANTIGEN: Included free of charge with the antibody is 30 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Product Information

Format

Affinity Purified

Control

CONTROL ANTIGEN: Included free of charge with the antibody is 30 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	This Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, C-terminus is validated for use in IC, IH, WB for the detection of Endothelin Receptor A.
Key Applications	Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:200 using ECL on rat brain membranes. Detects a major band at ~50KDa, with occassional lower molecular weight bands. Immunohistochemistry Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 mL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h) 3. Transfer in semi-dry system under standard conditions (3 h 200 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 15 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 15 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).

Applications

Application

This Anti-Endothelin Receptor A Antibody, a.a. 413-426 rET-A, C-terminus is validated for use in IC, IH, WB for the detection of Endothelin Receptor A.

Key Applications

Immunocytochemistry
Immunohistochemistry
Western Blotting

Application Notes

Western blot: 1:200 using ECL on rat brain membranes. Detects a major band at ~50KDa, with occassional lower molecular weight bands.

Immunohistochemistry

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

SUGGESTED WESTERN BLOT PROTOCOL

1. Mix the samples with sample-buffer X 2, and heat 10 min at 70°C.

2. 5-50 mL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h)

3. Transfer in semi-dry system under standard conditions (3 h 200 mA for two minigel gels)

4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

5. Destain with deionized water.

6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

8. Wash 4 x 15 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

10. Wash 4 x 15 min. with PBS-0.1% tween 20.

11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).

Biological Information
Immunogen	Purified peptide corresponding to amino acids 413-426 of the rat Endothelin Receptor A (Accession P26684).
Epitope	a.a. 413-426 rET-A, C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Endothelin Receptor A (ET-A). The immunogen sequence is specific for ET-A and is not present in any other known protein. The antibody is not expected to cross with ET-B but it has not been specifically tested. SPECIES REACTIVITIES: The immunogen sequence is highly conserved in human, porcine, bovine (13/14 residues) and chicken (12/14). Reactivity with other species has not been confirmed.
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001957.1
Gene Symbol	EDNRA ETRA ETA ET-A ETA-R
UniProt Number	P25101
UniProt Summary	FUNCTION: SwissProt: P25101 # Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 > ET3. SIZE: 427 amino acids; 48722 Da SUBUNIT: Interacts with HDAC7 and HTATIP. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. SIMILARITY: SwissProt: P25101 ## Belongs to the G-protein coupled receptor 1 family.

Biological Information

Immunogen

Purified peptide corresponding to amino acids 413-426 of the rat Endothelin Receptor A (Accession P26684).

Epitope

a.a. 413-426 rET-A, C-terminus

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Endothelin Receptor A (ET-A). The immunogen sequence is specific for ET-A and is not present in any other known protein. The antibody is not expected to cross with ET-B but it has not been specifically tested.

SPECIES REACTIVITIES: The immunogen sequence is highly conserved in human, porcine, bovine (13/14 residues) and chicken (12/14). Reactivity with other species has not been confirmed.

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_001957.1

Gene Symbol

EDNRA
ETRA
ETA
ET-A
ETA-R

UniProt Number

P25101

UniProt Summary

FUNCTION: SwissProt: P25101 # Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 > ET3.
SIZE: 427 amino acids; 48722 Da
SUBUNIT: Interacts with HDAC7 and HTATIP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
SIMILARITY: SwissProt: P25101 ## Belongs to the G-protein coupled receptor 1 family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Rb X Endothelin Receptor A

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