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Rb Anti-K+ CI- Cotransporter-4

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货号：AB3270

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3270
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-K+-Cl- Cotransporter 4 Antibody
Alternate Names
  • KCC4
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide.
Applications
Application Anti-K+-Cl-Cotransporter 4 Antibody is an antibody against K+-Cl-Cotransporter 4 for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL using ECL.

Approximate size on reduced westerns is 120-140kDa; membrane preparations and proteinase inhibitors are strongly recommended. See protocol under Technical Service as www.chemicon.com.



ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A 16 amino acid peptide within the cytoplasmic, N-terminus of mouse KCC4.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes K+ Cl- Cotransporter-4 (KCC4). The immunogen shows no significant sequence homology with other KCCs or CCC.

SPECIES REACTIVITIES: The immunogen sequence is 81% homologous with human KCC4. Reactivity with other species has not been confirmed.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC12A7
  • KCC4
  • DKFZP434F076
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9Y666 # Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).
SIZE: 1083 amino acids; 119106 Da
SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Detected in muscle, brain, lung, heart and kidney.
SIMILARITY: SwissProt: Q9Y666 ## Belongs to the SLC12A transporter family.
MISCELLANEOUS: Activated by N-ethylmaleimide (NEM). Inhibited by furosemide, DIDS and bumetanide. The inhibition is much stronger in the presence of 50 mM K(+) in the uptake medium. Inhibited by DIOA.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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