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Rb X Aquaporin 1

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0.00
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¥0.00

货号：AB3272-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R IHC, WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB3272-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Aquaporin 1 Antibody
Alternate Names
  • AQP1
  • CHIP28
References
Product Information
Format Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Applications
Application Anti-Aquaporin 1 Antibody is an antibody against Aquaporin 1 for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blotting: 1:400-1:800 using ECL on rat kidney membranes.

Immunohistochemistry

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Highly purified peptide corresponding to residues 242-260 of human Aquaporin 1 (SwissProt Accession # P29972).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Aquaporin 1.

SPECIES REACTIVITIES: The epitope specific for Aquaporin 1 is identical in human, rodent, bovine and only slightly different in frog. Reactivity with other species has not yet been tested.
Species Reactivity
  • Human
  • Rat
Species Reactivity Note The epitope specific for Aquaporin 1 is identical in rodent, bovine, sheep and only slightly different in frog (Rana esculenta) (18/19).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement.
Gene Symbol
  • AQP1
  • Aquaporin-CHIP
  • MGC26324
  • AQP-CHIP
  • CO
  • Aquaporin-1
  • CHIP28
  • AQP-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P29972 # Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 269 amino acids; 28526 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
DOMAIN: SwissProt: P29972 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
MISCELLANEOUS: Pharmacologically inhibited by submillimolar concentrations of mercury.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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