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Rabbit Anti-Aquaporin 2

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号:AB3274-50UL

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R WB, IHC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3274-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Aquaporin 2 Antibody
Alternate Names
  • AQP2
  • AQP-CD
  • WCD-CD
References
Product Information
Format Affinity Purified
Control
  • Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Applications
Application Anti-Aquaporin 2 Antibody detects level of Aquaporin 2 & has been published & validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Western blotting: 1.0-3.0 μg/mL (1:200-1:600 using ECL) on rat kidney membranes.

Immunohistochemistry

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Highly purified peptide corresponding to residues 254-271 of rat or mouse Aquaporin 21,2 with an additional N-terminal lysine and tyrosine.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Aquaporin 2.

SPECIES REACTIVITIES: The epitope specific for Aquaporin 2 is identical in rat and mouse and only slightly different in human (17/18 amino acids). Reactivity with other species has not yet been tested.
Species Reactivity
  • Human
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus.
Gene Symbol
  • AQP2
  • AQP-CD
  • aquaporin-CD
  • Aquaporin-2
  • Aquaporin-CD
  • AQP-2
  • MGC34501
  • WCH-CD
UniProt Number
UniProt Summary FUNCTION: SwissProt: P41181 # Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 271 amino acids; 28837 Da
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane.
TISSUE SPECIFICITY: Expressed in renal collecting tubules.
DOMAIN: SwissProt: P41181 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
PTM: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
DISEASE:SwissProt: P41181 # Defects in AQP2 are a cause of autosomal nephrogenic diabetes insipidus, type I (AD-NDI) [MIM:125800]. It is characterized by excessive water drinking (polydypsia) and urine excretion (polyuria).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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