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Anti-CHD-1 (rabbit polyclonal)

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货号：06-1339

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 06-1339
Description Anti-CHD-1 Antibody
Alternate Names
  • chromodomain helicase DNA binding protein 1
  • ATP-dependent helicase CHD1
  • chromodomain-helicase-DNA-binding protein 1
Background Information Chromodomain-helicase-DNA-binding protein 1 (CHD-1) is a member of the SNF2/RAD54 helicase family and is comprised of two chromo domains, one helicase ATP-binding domain, and one helicase C-terminal domain. CHD-1 is a regulator for RNA polymerase I transcription, polymerase II transcription, and a negative regulator for DNA replication. It is a chromatin-remodeling factor dependent on ATP and acting as a component for substrate recognition in HAT complex SAGA transcription. Studies have demonstrated CHD-1 involvement in HDAC activity and in the regulation of pre-mRNA splicing efficacy. CHD-1 is a required component in the maintenance of embryonic stem (ES) cell pluripotency.
References
Product Information
Format Affinity Purified
Control
  • HeLa nuclear extract
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-CHD-1 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-1 also known as chromodomain helicase DNA binding protein 1, ATP-dependent helicase CHD1 & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected CHD-1 in NIH/3T3 and HeLa cells.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human CHD-1.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Demonstrated to react with human and mouse.
Other homologies: Rat (91% sequence homology). Chimpanzee (99% sequence homology).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. [provided by RefSeq].
Gene Symbol
  • CHD1
  • CHD-1
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells. Ref.10

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Component of the SAGA complex (By similarity). Interacts with BCLAF1, NCoR, SRP20 and SAFB By similarity. Specifically interacts with methylated H3K4me2 and H3K4me3. Interacts with the FACT complex, the PAF complex and the U2 snRNP. Interacts directly with PAF1, SFA3A1, SFA3A2, SFA3A3, SNF2 and SSRP1.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). Note: Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis (By similarity).

DOMAIN: The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3). Ref.8

SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

Contains 2 chromo domains.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.
Molecular Weight ~170 kDa observed. Calculated MW ~197 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.25 µg/mL of this antibody detected CHD-1 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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