Rb Anti-Hu DAP12

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Polyclonal Antibody

Description
Catalogue Number	AB3890
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-DAP12 Antibody
Background Information	Natural killer (NK) cells are lymphocytes that function as a component of innate immunity against intracellular and parasitic pathogens, and possibly tumors. DAP12 is expressed on the surface of NK cells as a disulfide-linked homodimer and contains an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. DAP12 non-covalently associates with members of the killer cell immunoglobulin-like receptor (KIR) family resulting in cellular activation.

Description

Catalogue Number

AB3890

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-DAP12 Antibody

Background Information

Natural killer (NK) cells are lymphocytes that function as a component of innate immunity against intracellular and parasitic pathogens, and possibly tumors. DAP12 is expressed on the surface of NK cells as a disulfide-linked homodimer and contains an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. DAP12 non-covalently associates with members of the killer cell immunoglobulin-like receptor (KIR) family resulting in cellular activation.

Product Information
Format	Purified
Control	Western blot positive control: U937 cell line
Presentation	Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide.

Product Information

Format

Purified

Control

Western blot positive control: U937 cell line

Presentation

Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide.

Applications
Application	Anti-DAP12 Antibody is an antibody against DAP12 for use in WB.
Key Applications	Western Blotting
Application Notes	Western blot: 1.0 μg/mL Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-DAP12 Antibody is an antibody against DAP12 for use in WB.

Key Applications

Western Blotting

Application Notes

Western blot: 1.0 μg/mL

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide corresponding to amino acid residues 89-113 of human DAP12 (Accession # AAD09437).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes human DAP12. This antibody detects a band of approximately 12 kDa in Western blot analysis of U937 cell lysate.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_003332.2 NM_198125.1
Entrez Gene Summary	This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined.
Gene Symbol	TYROBP DAP12 PLO-SL KARAP PLOSL
UniProt Number	O43914
UniProt Summary	FUNCTION: SwissProt: O43914 # Non-covalently associates with membrane glycoproteins of the killer-cell inhibitory receptor (KIR) family without an ITIM in their cytoplasmic domain. Cross-linking of KIR-TYROBP complexes results in cellular activation. SIZE: 113 amino acids; 12179 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with CLECSF5. Interacts with SIGLEC14. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum. PTM: Tyrosine phosphorylated. DISEASE: "SwissProt: O43914 # Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns." SIMILARITY: SwissProt: O43914 ## Belongs to the TYROBP family.

Biological Information

Immunogen

Synthetic peptide corresponding to amino acid residues 89-113 of human DAP12 (Accession # AAD09437).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes human DAP12. This antibody detects a band of approximately 12 kDa in Western blot analysis of U937 cell lysate.

Species Reactivity

Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined.

Gene Symbol

TYROBP
DAP12
PLO-SL
KARAP
PLOSL

UniProt Number

O43914

UniProt Summary

FUNCTION: SwissProt: O43914 # Non-covalently associates with membrane glycoproteins of the killer-cell inhibitory receptor (KIR) family without an ITIM in their cytoplasmic domain. Cross-linking of KIR-TYROBP complexes results in cellular activation.
SIZE: 113 amino acids; 12179 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with CLECSF5. Interacts with SIGLEC14.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
PTM: Tyrosine phosphorylated.
DISEASE: "SwissProt: O43914 # Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns."
SIMILARITY: SwissProt: O43914 ## Belongs to the TYROBP family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C for up to 12 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain at 2-8°C for up to 12 months from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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