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Rabbit Anti-Dopamine Polyclonal Antibody

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号:AB122S

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB122S
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Dopamine Antibody
References
Product Information
Format Serum
Presentation Neat rabbit antisera with 0.1% sodium azide.
Applications
Application Detect Dopamine using this Anti-Dopamine Antibody validated for use in IH.
Key Applications
  • Immunohistochemistry
Application Notes Immunohistochemistry at 1:500-1:1,000.

PERFUSION MIXTURE AND PROTOCOL FOR IMMUNOCYTOCHEMICAL DETECTION OF DOPAMINE: See suggested protocols.

AB122S should be used at dilutions of 1:500-1:1,000 using Tris-MBS containing 0.5% Trition and 1% normal goat serum. The antibody should be incubated 15 hours at +4°C or room temperature. Final working dilutions must be determined by end user.

Post Treatment:

The immunocytochemistry procedure is then continued with the PAP method or the avidin-biotin peroxidase system. Note: since MBS is known to inhibit PAP or avidin-biotin peroxidase, it should be omitted for this step. 40 mM Tris-HCl with 9% NaCl adjusted to pH 7.6 (using concentrated HCl) should be used.

Notations:

MBS is essential in the pre-wash and the glutaraldehyde solution since this antioxidant protects the dopamine molecule from being released.

*Protocol modified from: McRae-Degueurce, A., et al. (1986). Brain Research 376:217-219.
Biological Information
Immunogen Dopamine-BSA-Glutaraldehyde conjugate
Host Rabbit
Specificity Dopaminergic neurons of substantia nigra and of the A10 region as well as dopaminergic nerve terminals of locus coeruleus. Immunostaining is abolished by pre-incubation with 10-100 μg dopamine-BSA-Glutaraldehyde conjugate per mL of diluted antibody. Repeated absorptions of the serum with nonadrenaline coupled to BSA does not alter the immunocytochemical staining pattern.
Species Reactivity
  • Human
  • Rat
Antibody Type Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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