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Ms X Dopamine D1b (D5) Receptor

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货号：MAB5292

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R IHC, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB5292
Replaces AB1791P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B
Alternate Names
  • Dopamine D5 Receptor
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Applications
Application This Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B is validated for use in IH, WB for the detection of Dopamine D1B Receptor.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:250-1:500 on mouse brain lysate (unboiled sample)

Immunohistochemistry

Optimal working dilutions must be determined by the end user.

SUGGESTED WESTERN BLOT PROTOCOL

Solutions

10X Transfer buffer 1 liter

Tris 115.3 gm

Glycine 24.2 gm

[SDS 4 gm (may be omitted)]

For a 1X working solution: 1 liter

mix 700 mL water

200 mL

MeOH100 mL

10X Transfer

TBSTstock 1 liter1M

Tris pH 7.5 10 mL

5 M NaCl 30 mL

10% Tween-20 5 Ml

1. Run SDS-PAGE gel as desired.

2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.

3. Soak membrane in transfer buffer (see below) until ready to set up blot (>15 min) transfer.

4. Assemble gel-transfer sandwich with the blot toward the positive electrode.

5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.

6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

7. Destain with deionized water.

8. Block with 5% non-fat milk (Marvel or Carnation) in water, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

9. Incubation with first antibody overnight with rocking at 4°C in blocking solution. Optimal working dilutions and incubation time will need to be determined by the end user.

10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.

11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.

12. Wash at least 3 x 5 min. with TBST.

13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
Biological Information
Immunogen Recombinant rat D1b Receptor, carboxy terminus.
Clone SG4-D1B
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Dopamine Receptor D1b (D5).
Isotype IgG1
Species Reactivity
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2.
Gene Symbol
  • DRD5
  • DRD1B
  • DRD1L2
  • DBDR
  • D
  • MGC10601
Non-Reactive Species
  • Human
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 477 amino acids; 52951 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.
DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.
SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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