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Rb X AT2 Receptor

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货号：AB15554-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB15554-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-AT2 Receptor Antibody
Alternate Names
  • Angiotensin II receptory type 2
  • AGTR2
References
Product Information
Format Affinity Purified
Control
  • Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application This Anti-AT2 Receptor Antibody is validated for use in WB for the detection of AT2 Receptor.
Key Applications
  • Western Blotting
Application Notes Western blot: 1:200-1:500 using ECL on rat brain and mouse MS1 cell lysate.
Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified peptide from the N-terminus of rat AT2 Receptor (Accession number P35351).
Host Rabbit
Specificity Recognizes AT2 Receptor (Angiotensin II receptory type 2, AGTR2).
Species Reactivity
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G-protein coupled receptors. It is an intergral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame.
Gene Symbol
  • AGTR2
  • MRX88
  • AT2
  • ATGR2
Non-Reactive Species
  • Human
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P50052 # Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
SIZE: 363 amino acids; 41184 Da
SUBUNIT: Interacts with MTUS1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
SIMILARITY: SwissProt: P50052 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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