Anti-SorLA, clone 20C11 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	ICC, WB, ELISA	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

ICC, WB, ELISA

Purified

Monoclonal Antibody

Description
Catalogue Number	MABN1793
Description	Anti-SorLA Antibody, clone 20C11
Alternate Names	Sortilin-related receptor Gp250 LDLR relative with 11 ligand-binding repeats Low-density lipoprotein receptor relative with 11 ligand-binding repeats LR11 SorLA SorLA-1 Sorting protein-related receptor containing LDLR class A repeats
Background Information	Sortilin-related receptor (UniProt Q92673; also known as Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats) is encoded by the SORL1 (also known as C11orf32) gene (Gene ID 6653) in human. SorLA and Sortilin are related Vps10p-domain (Vsp10p-D) family multi-ligand-binding receptors that target LPL, growth factors and peptides, as well as soluble amyloid precursor proein (sAPP) for endocytosis. Studies show that SorLA protects sAPP against proteolytic processing in compartments localized in the cell body (soma) of hippocampal neurons, whereas sortilin facilitates sAPP degradation by lysosomes localized in neurites. Hippocampal neurons lacking sortilin display reduced sAPPα production, suggesting that sortilin promotes α-secretase activity in neuronal systems. Unlike LRP1 and LRP1b, two other known sAPP-binding receptors, SorLA and Sortilin bind sAPP in a KPI domain-independent manner and their scavenging function therefore is not limited to sAPP produced from KPI-containing APP isoforms. Human SorLA is a single transmembrane (a.a. 2138-2158) protein with a 55-amino acid cytoplasmic tail (a.a. 2159-2214) and a large luminal/extracellular (a.a. 1-2137) region that contains a Vps10p-domain (Vsp10p-D; a.a. 1-731) with a signal peptide (a.a. 1-28) and a propeptide (a.a. 29-81) sequence at the N-terminal end and five BNR (bacterial neuraminidase repeat or (F/Y)WTD/b-propeller domain) repeats (a.a. 136-573). The C-terminal half of the luminal/extracellular region contains five LDL-R class B (LB) repeats (a.a. 800-1013), an EGF-like domain (a.a. 1026-1072), eleven LDL-R class A (LA) repeats (a.a. 1076-1551), and six fibronectin type-III repeats (a.a. 1557-2118).

Description

Catalogue Number

MABN1793

Description

Anti-SorLA Antibody, clone 20C11

Alternate Names

Sortilin-related receptor
Gp250
LDLR relative with 11 ligand-binding repeats
Low-density lipoprotein receptor relative with 11 ligand-binding repeats
LR11
SorLA
SorLA-1
Sorting protein-related receptor containing LDLR class A repeats

Background Information

Sortilin-related receptor (UniProt Q92673; also known as Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats) is encoded by the SORL1 (also known as C11orf32) gene (Gene ID 6653) in human. SorLA and Sortilin are related Vps10p-domain (Vsp10p-D) family multi-ligand-binding receptors that target LPL, growth factors and peptides, as well as soluble amyloid precursor proein (sAPP) for endocytosis. Studies show that SorLA protects sAPP against proteolytic processing in compartments localized in the cell body (soma) of hippocampal neurons, whereas sortilin facilitates sAPP degradation by lysosomes localized in neurites. Hippocampal neurons lacking sortilin display reduced sAPPα production, suggesting that sortilin promotes α-secretase activity in neuronal systems. Unlike LRP1 and LRP1b, two other known sAPP-binding receptors, SorLA and Sortilin bind sAPP in a KPI domain-independent manner and their scavenging function therefore is not limited to sAPP produced from KPI-containing APP isoforms. Human SorLA is a single transmembrane (a.a. 2138-2158) protein with a 55-amino acid cytoplasmic tail (a.a. 2159-2214) and a large luminal/extracellular (a.a. 1-2137) region that contains a Vps10p-domain (Vsp10p-D; a.a. 1-731) with a signal peptide (a.a. 1-28) and a propeptide (a.a. 29-81) sequence at the N-terminal end and five BNR (bacterial neuraminidase repeat or (F/Y)WTD/b-propeller domain) repeats (a.a. 136-573). The C-terminal half of the luminal/extracellular region contains five LDL-R class B (LB) repeats (a.a. 800-1013), an EGF-like domain (a.a. 1026-1072), eleven LDL-R class A (LA) repeats (a.a. 1076-1551), and six fibronectin type-III repeats (a.a. 1557-2118).

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Presentation

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-SorLA Antibody, clone 20C11 is an antibody against SorLA for use in Immunocytochemistry, Western Blotting, ELISA.
Key Applications	Immunocytochemistry Western Blotting ELISA
Application Notes	Immunocytochemistry Analysis: 5 µg/mL from a representative lot immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes (Courtesy of Dr. Jakob V. Larsen, MIND-centre, University of Aarhus, Denmark). Immunocytochemistry Analysis: A representative lot detected SorLA immunoreactivity primarily located at the cell body (soma) by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized primary murine hippocampal neurons (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71). Immunocytochemistry Analysis: A representative lot immunostained endocytic vesicular structures containing endocytosed sAPP by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells expressing exogenously transfected human SorLA (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71). Immunocytochemistry Analysis: A representative lot detected the cellular localization of exogenously expressed wild-type and the FANSHY-to-6A4 mutant human SorLA by fluorescent immuncytochemistry staining of 4% paraformaldehyde-fixed, 0.5% saponin-permeabilized SH-SY5Y transfectants (Fjorback, A.W., et al. (2012). J. Neurosci. 32(4):1467-1480). Western Blotting Analysis: A representative lot detected endogenous SorLA in sucrose gradient-fractionated mouse brain extracts as well as the expression of exogenously transfected human SorLA in HEK293 transfectants (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71). ELISA Analysis: Representative lots were employed as the detection antibody for quantiating SorLA level in human brain cortical extracts as well as the level of exogenously expressed human SorLA in CHO cells (Caglayan, S., et al. (2012). Arch. Neurol. 69(3):373-379; Schmidt, V., et al. (2012). EMBO J. 31(1):187-200).

Applications

Application

Anti-SorLA Antibody, clone 20C11 is an antibody against SorLA for use in Immunocytochemistry, Western Blotting, ELISA.

Key Applications

Immunocytochemistry
Western Blotting
ELISA

Application Notes

Immunocytochemistry Analysis: 5 µg/mL from a representative lot immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes (Courtesy of Dr. Jakob V. Larsen, MIND-centre, University of Aarhus, Denmark).
Immunocytochemistry Analysis: A representative lot detected SorLA immunoreactivity primarily located at the cell body (soma) by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized primary murine hippocampal neurons (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot immunostained endocytic vesicular structures containing endocytosed sAPP by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells expressing exogenously transfected human SorLA (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot detected the cellular localization of exogenously expressed wild-type and the FANSHY-to-6A4 mutant human SorLA by fluorescent immuncytochemistry staining of 4% paraformaldehyde-fixed, 0.5% saponin-permeabilized SH-SY5Y transfectants (Fjorback, A.W., et al. (2012). J. Neurosci. 32(4):1467-1480).
Western Blotting Analysis: A representative lot detected endogenous SorLA in sucrose gradient-fractionated mouse brain extracts as well as the expression of exogenously transfected human SorLA in HEK293 transfectants (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
ELISA Analysis: Representative lots were employed as the detection antibody for quantiating SorLA level in human brain cortical extracts as well as the level of exogenously expressed human SorLA in CHO cells (Caglayan, S., et al. (2012). Arch. Neurol. 69(3):373-379; Schmidt, V., et al. (2012). EMBO J. 31(1):187-200).

Biological Information
Immunogen	Recombinant human SorLA extracellular/luminal domain.
Epitope	Extracellular/luminal region outside the Vps10p domain (Vsp10p-D).
Clone	20C11
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 20C11 immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes.
Isotype	IgG1κ
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003096
Gene Symbol	SORL1 C11orf32
Purification Method	Protein G Purified
UniProt Number	Q92673
Molecular Weight	239.3 kDa (human) and 238.3 kDa (mouse) calculated.

Biological Information

Immunogen

Recombinant human SorLA extracellular/luminal domain.

Epitope

Extracellular/luminal region outside the Vps10p domain (Vsp10p-D).

Clone

20C11

Concentration

Please refer to lot specific datasheet.

Host

Mouse

Specificity

Clone 20C11 immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes.

Isotype

IgG1κ

Species Reactivity

Human
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_003096

Gene Symbol

SORL1
C11orf32

Purification Method

Protein G Purified

UniProt Number

Q92673

Molecular Weight

239.3 kDa (human) and 238.3 kDa (mouse) calculated.

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in human SorLA-expressing HEK293 cells. Immunocytochemistry Analysis: 4.0 µg/mL of this antibody immunostained SorLA endocytic vesicles in HEK293 cells expressing transfected human SorLA.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Immunocytochemistry in human SorLA-expressing HEK293 cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody immunostained SorLA endocytic vesicles in HEK293 cells expressing transfected human SorLA.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Packaging Information

Material Size

100 μg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-SorLA, clone 20C11 (mouse monoclonal)

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