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Gt X GlutamateTransporter (EAAC1)

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货号：AB1520

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Gp, M, R IHC Gt Serum Polyclonal Antibody
Description
Catalogue Number AB1520
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Glutamate Transporter Antibody, neuronal
Alternate Names
  • EAAC1
  • EAAT3
References
Product Information
Format Serum
Presentation Goat antiserum. Liquid, no preservatives.
Applications
Application Anti-Glutamate Transporter Antibody, neuronal detects level of Glutamate Transporter & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Applications Not Recommended
  • Western Blotting
Application Notes Immunohistochemistry: 1:4,000-8,000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material is recommended. The use of triton X-100 in the block only is recommended.

This antibody is not recommended for use in Western blot.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide from the carboxy-terminus of the cloned rat EAAC1.
Host Goat
Specificity Neuronal Glutamate Transporter (EAAC1; also called EAAT3). The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained with AB1520 corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preabsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining.
Species Reactivity
  • Guinea Pig
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC1A1
  • EAAC1
  • EAAT3
UniProt Number
UniProt Summary FUNCTION: SwissProt: P43005 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 (By similarity).
SIZE: 524 amino acids; 57100 Da
SUBUNIT: Interacts with ARL6IP5/PRAF3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
PTM: Glycosylated.
DISEASE: SwissProt: P43005 # Defects in SLC1A1 may be a cause of dicarboxylicamino aciduria [MIM:222730]; also known as glutamate-aspartate transport defect. This is as defect in renal and probably intestinal transport of glutamic and aspartic acids and is associated with moderate hyperprolinemia.
SIMILARITY: SwissProt: P43005 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to one year.

Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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