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Ms X Hu Alpha Actinin

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货号：MAB1682

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IF, IP, WB M Ascites Monoclonal Antibody
Description
Catalogue Number MAB1682
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Actinin α Antibody, clone AT6/172
References
Product Information
Format Ascites
Presentation Liquid
Applications
Application Anti-Actinin Antibody, α, clone AT6/172 detects level of Actinin & has been published & validated for use in IF, IP & WB.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoblotting: ~100kDa on SDS reducing gels

Immunoprecipitation

Immunofluorescence: methanol fixed cells

Immunohistochemistry



Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Human platelet protein
Epitope alpha
Clone AT6/172
Host Mouse
Specificity Human alpha actinin. This antibody recognizes an epitope in the N-terminal region of alpha-actinin, probably between residues 1-24.
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Gene Symbol
  • ACTN1
  • FLJ40884
  • Alpha-actinin-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P12814 # F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
SIZE: 892 amino acids; 103058 Da
SUBUNIT: Homodimer; antiparallel. Interacts with MYOZ2, PDLIM2 and TTID and LPP.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z-disk. Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
SIMILARITY: SwissProt: P12814 ## Belongs to the alpha-actinin family. & Contains 1 actin-binding domain. & Contains 2 CH (calponin-homology) domains. & Contains 2 EF-hand domains. & Contains 4 spectrin repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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