Ms X Protein Kinase II, cAMP-Dependent Regulatory Subunit

Species Reactivity	Key Applications	Host	Format	Antibody Type
Ma, H	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Ma, H

WB, IHC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	07-1493
Replaces	AB1697
Description	Anti-Calcineurin Aβ Antibody
Alternate Names	NFAT transcription complex cytosolic component nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 nuclear factor of activated T-cells, cytosolic component 1
Background Information	Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin's multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Description

Catalogue Number

07-1493

Replaces

AB1697

Description

Anti-Calcineurin Aβ Antibody

Alternate Names

NFAT transcription complex cytosolic component
nuclear factor of activated T-cells, cytoplasmic,
calcineurin-dependent 1
nuclear factor of activated T-cells, cytosolic component 1

Background Information

Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin's multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Product Information
Format	Affinity Purified
Control	Human brain lysate
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Product Information

Format

Affinity Purified

Control

Human brain lysate

Presentation

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Applications
Application	Detect Calcineurin Aβ using this Anti-Calcineurin Aβ Antibody validated for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry

Applications

Application

Detect Calcineurin Aβ using this Anti-Calcineurin Aβ Antibody validated for use in WB, IH.

Key Applications

Western Blotting
Immunohistochemistry

Biological Information
Immunogen	KLH-conjugagted synthetic peptide corresponding to Calcineurin Aβ.
Host	Rabbit
Specificity	Recognizes the Aβ (polyproline) form of calcineurin, a less abundant gene isoform of calcineurin in most tissues. Allows differentiation of Aβ from the A α and A-γ isoforms. Reacts with calcineurin of all mammalian species.
Species Reactivity	Mammals Human
Species Reactivity Note	Proven to react with human. Predicted to react with all mammals based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_000935.1
Gene Symbol	MGC138448 NF-ATC NF-ATc NF-ATc1 NFAT2 NFATC NFATc
Purification Method	Affinity Purfied
UniProt Number	O95644
UniProt Summary	FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. SUBUNIT STRUCTURE: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. TISSUE SPECIFICITY: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. INDUCTION: Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A. DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus. Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor. PTM: Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin. SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.
Molecular Weight	Approx. 58 kDa

Biological Information

Immunogen

KLH-conjugagted synthetic peptide corresponding to Calcineurin Aβ.

Host

Rabbit

Specificity

Recognizes the Aβ (polyproline) form of calcineurin, a less abundant gene isoform of calcineurin in most tissues. Allows differentiation of Aβ from the A α and A-γ isoforms. Reacts with calcineurin of all mammalian species.

Species Reactivity

Mammals
Human

Species Reactivity Note

Proven to react with human. Predicted to react with all mammals based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_000935.1

Gene Symbol

MGC138448
NF-ATC
NF-ATc
NF-ATc1
NFAT2
NFATC
NFATc

Purification Method

Affinity Purfied

UniProt Number

O95644

UniProt Summary

FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
SUBUNIT STRUCTURE: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
TISSUE SPECIFICITY: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
INDUCTION: Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A.
DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
PTM: Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.

Molecular Weight

Approx. 58 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human brain lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin Aβ on 10 µg of human brain lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin Aβ on 10 µg of human brain lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will remain unfrozen. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Ms X Protein Kinase II, cAMP-Dependent Regulatory Subunit

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