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Ms X Hu Clathrin

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货号：CBL188

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Am, B, H, Po, R WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number CBL188
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Clathrin Antibody, clone CHC5.9
References
Product Information
Format Purified
Control
  • Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Presentation The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
Applications
Application Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Marker for detection of receptor mediated endocytosis

Suitable for frozen sections

Western blotting KNOW SPECIES

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Coated vesicles (clathrin) of bovine brain.
Clone CHC5.9
Host Mouse
Specificity This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
Isotype IgM
Species Reactivity
  • Amphibian
  • Bovine
  • Human
  • Pig
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Gene Symbol
  • CLTC
  • CHC17
  • CLTCL2
  • Hc
  • CLH17
  • CLH-17
  • KIAA0034
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q00610 # Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
SIZE: 1675 amino acids; 191615 Da
SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Cytoplasmic side. Membrane, coated pit; Cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: Q00610 ## Belongs to the clathrin heavy chain family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C for up to 12 months from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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