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Rat X HSF I

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货号：MAB88078

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, Mk ELISA, IF, IP, WB R Purified Monoclonal Antibody
Description
Catalogue Number MAB88078
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Heat Shock Factor I Antibody, clone 10H8
Alternate Names
  • HSFI
References
Product Information
Format Purified
Control
  • POSITIVE CONTROL: NIH-3T3, K562, HL-60, HEP-2, or HeLa cells
Presentation Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
Applications
Application This Anti-Heat Shock Factor I Antibody, clone 10H8 is validated for use in ELISA, IF, IP, WB for the detection of Heat Shock Factor I.
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application Notes I mmunofluorescence (predominantly nuclear)

Immunoprecipitation: 10 μL/mg cell lysate

Western Blot: 1:200-1:400 (for 2hrs. at Room Temperature)

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Full length recombinanat mouse HSF1 protein (aa 1-503).
Clone 10H8
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Specificity MAB88078 recognizes a protein of 70-85 kDa (depending upon the souce and state of cells), identified as Heat Shock Factor 1 (HSF1). Reactivity with delection mutants suggests that the epitope of MAB88078 spans in the aa 378-395 of mouse HSF1. The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediate by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosophila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. The phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.

Cellular Localization: Cytoplasmic domain
Isotype IgG1
Species Reactivity
  • Human
  • Mouse
  • Monkey
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.
Gene Symbol
  • HSF1
  • HSTF1
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q00613 # DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
SIZE: 529 amino acids; 57260 Da
SUBUNIT: DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a monomer. Interacts with SYMPK and CSTF2 in heat-stressed cells.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.
SIMILARITY: SwissProt: Q00613 ## Belongs to the HSF family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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