Anti-Bim, cln 14A8 (rat monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	FC, IHC, IP, WB	R	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

FC, IHC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	MAB17001-50UG
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Bim Antibody, clone 14A8
Overview	Purity: The antibody is shown to be >95% pure as determined by SDS-PAGE. The BimS antibody is supplied 0.22 micron filtered.
Background Information	Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Description

Catalogue Number

MAB17001-50UG

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Bim Antibody, clone 14A8

Overview

Purity: The antibody is shown to be >95% pure as determined by SDS-PAGE. The BimS antibody is supplied 0.22 micron filtered.

Background Information

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Product Information
Format	Purified
Control	Skin/Skin Tumor
Presentation	Protein A Purified mouse immunoglobulin in PBS containing 0.05% Bronidox as preservative.

Product Information

Format

Purified

Control

Skin/Skin Tumor

Presentation

Protein A Purified mouse immunoglobulin in PBS containing 0.05% Bronidox as preservative.

Applications
Application	Anti-Bim Antibody, clone 14A8 is an antibody against Bim for use in FC, IH, IP & WB.
Key Applications	Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	The antibody is intended for the detection of all splice variants of Bim in a range of techniques including: Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details. immunoprecipitation FACS immunohistochemistry. It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.

Applications

Application

Anti-Bim Antibody, clone 14A8 is an antibody against Bim for use in FC, IH, IP & WB.

Key Applications

Flow Cytometry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

The antibody is intended for the detection of all splice variants of Bim in a range of techniques including:

Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.

immunoprecipitation

FACS

immunohistochemistry.

It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.

Biological Information
Immunogen	Recombinant mouse BimShort (BimS) protein.
Clone	14A8
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Specificity	Purified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.
Isotype	IgG2a
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000852.2
Entrez Gene Summary	Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Purification Method	Protein G Purified
UniProt Number	O43521
UniProt Summary	Function: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity. Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity. Isoform BimEL: Mitochondrion Isoform BimL: Mitochondrion Isoform BimS: Mitochondrion Isoform Bim-alpha1: Mitochondrion Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3 Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity. Sequence similarities: Belongs to the Bcl-2 family.
Molecular Weight	~17-23kDa

Biological Information

Immunogen

Recombinant mouse BimShort (BimS) protein.

Clone

14A8

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rat

Specificity

Purified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.

Isotype

IgG2a

Species Reactivity

Human
Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000852.2

Entrez Gene Summary

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Purification Method

Protein G Purified

UniProt Number

O43521

UniProt Summary

Function: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
Isoform BimEL: Mitochondrion
Isoform BimL: Mitochondrion
Isoform BimS: Mitochondrion
Isoform Bim-alpha1: Mitochondrion
Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities: Belongs to the Bcl-2 family.

Molecular Weight

~17-23kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Bim, cln 14A8 (rat monoclonal)

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询