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Ms X Ubiquitin Activating Enzyme E1

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货号：MAB3520

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M IP, WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB3520
Replaces 05-624
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Ubiquitin Activating Enzyme E1 Antibody, clone 2G2.3
Background Information The proteosome has long been thought of as the default mechanism for waste elimination within the cell. Recently, however, the proteosome has been recognized as an essential organelle necessary for the processing of antigens, the transcription factor NFkB and other proteins as well. Central to the function of the proteosome is the ubiquitinization of target proteins by a number of unique ubiquitin activation enzymes.
References
Product Information
Format Purified
Presentation Liquid. Buffer = 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide.
Applications
Application Anti-Ubiquitin Activating Enzyme E1 Antibody, clone 2G2.3 detects level of Ubiquitin Activating Enzyme E1 & has been published & validated for use in IP, WB, IH.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Application Notes Western blot: 2-4 μg/mL

Immunohistochemistry

Immunoprecipitation

Sero-depletion

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Ubiquitin activating enzyme E1.
Clone 2G2.3
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity The antibody specifically binds to ubiquitin activation enzyme E1. The antibody does not inhibit the enzymatic activity of E1.
Isotype IgG1
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Gene Symbol
  • UBA1
  • UBE1X
  • MGC4781
  • A1S9T
  • UBA1A
  • A1S9
  • UBE1
  • A1ST
  • GXP1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P22314 # Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
SIZE: 1058 amino acids; 117849 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family.
MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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