Anti-ZNT3 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Mk	WB, IHC, IF, EM	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Mk

WB, IHC, IF, EM

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABN994
Description	Anti-ZNT3 pAb Antibody
Alternate Names	Zinc transporter 3 Solute carrier family 30 member 3 ZnT-3
Background Information	Zinc transporter 3 (UniProt P97441; also known as Solute carrier family 30 member 3, ZnT-3) is encoded by the Slc30a3 (also known as Znt3) gene (Gene ID 22784) in murine species. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is reponsible for many chronic neurodegenerative diseases and acute neural injuries. Excessive and inadequate levels of bioavailable zinc are detrimental to the health of neurons. Two classes of multipass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis . These transporters are located on plasma and vesicular membranes with tissue-specific expression pattern. ZnT-3 is an output type Zn transporter expressed in brain, retina, and testis. All ten ZnTs that have been described share similar cellular topology containing 6 transmembrane domains (a.a. 76-96, 106-126, 146-166, 178-198, 236-256, 264-284 of murine ZnT-3), 4 cytoplasmic and 3 vacuolar domains, having both N- and C-terminal ends (a.a. 1-75 and 285-388 of murine ZnT-3) exposed at the cytoplasmic side, except for ZnT-5 which has extra 10 predicted transmembrane domains at the N-terminal end.

Description

Catalogue Number

ABN994

Description

Anti-ZNT3 pAb Antibody

Alternate Names

Zinc transporter 3
Solute carrier family 30 member 3
ZnT-3

Background Information

Zinc transporter 3 (UniProt P97441; also known as Solute carrier family 30 member 3, ZnT-3) is encoded by the Slc30a3 (also known as Znt3) gene (Gene ID 22784) in murine species. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is reponsible for many chronic neurodegenerative diseases and acute neural injuries. Excessive and inadequate levels of bioavailable zinc are detrimental to the health of neurons. Two classes of multipass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis . These transporters are located on plasma and vesicular membranes with tissue-specific expression pattern. ZnT-3 is an output type Zn transporter expressed in brain, retina, and testis. All ten ZnTs that have been described share similar cellular topology containing 6 transmembrane domains (a.a. 76-96, 106-126, 146-166, 178-198, 236-256, 264-284 of murine ZnT-3), 4 cytoplasmic and 3 vacuolar domains, having both N- and C-terminal ends (a.a. 1-75 and 285-388 of murine ZnT-3) exposed at the cytoplasmic side, except for ZnT-5 which has extra 10 predicted transmembrane domains at the N-terminal end.

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-ZNT3 pAb Antibody is an antibody against ZNT3 pAb for use in Western Blotting, Immunohistochemistry, Immunofluorescence, Electron Microscopy.
Key Applications	Western Blotting Immunohistochemistry Immunofluorescence Electron Microscopy
Application Notes	Western Blotting Analysis: A representative lot detected an upregulated ZnT-3 level in hippocampus tissue lysates from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309). Western Blotting Analysis: A representative lot detected ZnT-3 expression in brain lysates from wild-type, but not Znt3-knockout mice, while reduced ZnT-3 level was seen in brain tissues from Znt3+/- mice (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121). Western Blotting Analysis: A representative lot detected exogenously expressed murine ZnT-3 in transfected BHK baby hamster kidney fibroblasts and endogenous ZnT-3 in mouse brain, but not testis or kidney, tissue homogenates. No cross-reactivity was seen toward murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939). Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309). Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in brain regions of wild-type, but not Znt3-knockout mice, while reduced ZnT-3 immunoreactivity was seen in brain tissues from Znt3+/- mice using 4% paraformaldehyde-fixed frozen brain sections (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121). Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the zinc-containing mossy fiber (MF) projections in mouse and monkey hippocampus using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681). Immunohistochemistry Analysis: A representative lot immunostained Zn-rich mossy fiber projections emanating from dentate gyrus granule cell neurons as well as projections from hippocampal and cortical neurons in 4% paraformaldehyde-fixed frozen mouse brain sections (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939). Immunofluorescence Analysis: A representative lot immunostained mossy fiber (MF) sprouting in the hippocampus inner molecular layer (IML) by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed free-floating brain sections from pilocarpine-induced epileptic mice with or without medial ganglionic eminence (MGE) progenitor cell grafts (Hunt, R.F., et al. (2013). Nat. Neurosci. 16(6):692-697). Immunofluorescence Analysis: A representative lot detected ZnT-3 immunoreactivity in Aβ-positive senile plaques (SP) by dual fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed, paraffin-embedded Alzheimer's diseased human cerebral cortex sections (Zhang, L.H., et al. (2008). Brain Res. Bull. 77(1):55-60). Electron Microscopy: A representative lot immunostained mossy fiber bouton (MFB) in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed frozen brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309). Electron Microscopy: A representative lot detected ZnT-3 immunoreactivity at the terminals of glutamatergic projections in mouse and monkey hippocampus by EM using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681).

Applications

Application

Anti-ZNT3 pAb Antibody is an antibody against ZNT3 pAb for use in Western Blotting, Immunohistochemistry, Immunofluorescence, Electron Microscopy.

Key Applications

Western Blotting
Immunohistochemistry
Immunofluorescence
Electron Microscopy

Application Notes

Western Blotting Analysis: A representative lot detected an upregulated ZnT-3 level in hippocampus tissue lysates from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Western Blotting Analysis: A representative lot detected ZnT-3 expression in brain lysates from wild-type, but not Znt3-knockout mice, while reduced ZnT-3 level was seen in brain tissues from Znt3+/- mice (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).
Western Blotting Analysis: A representative lot detected exogenously expressed murine ZnT-3 in transfected BHK baby hamster kidney fibroblasts and endogenous ZnT-3 in mouse brain, but not testis or kidney, tissue homogenates. No cross-reactivity was seen toward murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in brain regions of wild-type, but not Znt3-knockout mice, while reduced ZnT-3 immunoreactivity was seen in brain tissues from Znt3+/- mice using 4% paraformaldehyde-fixed frozen brain sections (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the zinc-containing mossy fiber (MF) projections in mouse and monkey hippocampus using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681).
Immunohistochemistry Analysis: A representative lot immunostained Zn-rich mossy fiber projections emanating from dentate gyrus granule cell neurons as well as projections from hippocampal and cortical neurons in 4% paraformaldehyde-fixed frozen mouse brain sections (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939).
Immunofluorescence Analysis: A representative lot immunostained mossy fiber (MF) sprouting in the hippocampus inner molecular layer (IML) by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed free-floating brain sections from pilocarpine-induced epileptic mice with or without medial ganglionic eminence (MGE) progenitor cell grafts (Hunt, R.F., et al. (2013). Nat. Neurosci. 16(6):692-697).
Immunofluorescence Analysis: A representative lot detected ZnT-3 immunoreactivity in Aβ-positive senile plaques (SP) by dual fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed, paraffin-embedded Alzheimer's diseased human cerebral cortex sections (Zhang, L.H., et al. (2008). Brain Res. Bull. 77(1):55-60).
Electron Microscopy: A representative lot immunostained mossy fiber bouton (MFB) in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed frozen brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Electron Microscopy: A representative lot detected ZnT-3 immunoreactivity at the terminals of glutamatergic projections in mouse and monkey hippocampus by EM using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681).

Biological Information
Immunogen	MBP-tagged recombinant mouse ZnT-3 C-terminal fragment.
Epitope	C-terminal cytoplasmic tail.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This polyclonal antibody specifically detected exogenously expressed murine ZnT-3 with no cross-reactivity toward exogenously expressed murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939). This polyclonal antibody specifically detected ZnT-3 expression in wild-type, but not Znt3-knockout, mouse brain tissues (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).
Species Reactivity	Human Mouse Monkey
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_035903
Gene Symbol	Slc30a3 Znt3
Purification Method	Affinity Purfied
UniProt Number	P97441
Molecular Weight	~45 kDa observed. 41.82 kDa (mouse) and 41.95 kDa (human) calculated.

Biological Information

Immunogen

MBP-tagged recombinant mouse ZnT-3 C-terminal fragment.

Epitope

C-terminal cytoplasmic tail.

Concentration

Please refer to lot specific datasheet.

Host

Rabbit

Specificity

This polyclonal antibody specifically detected exogenously expressed murine ZnT-3 with no cross-reactivity toward exogenously expressed murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939). This polyclonal antibody specifically detected ZnT-3 expression in wild-type, but not Znt3-knockout, mouse brain tissues (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).

Species Reactivity

Human
Mouse
Monkey

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_035903

Gene Symbol

Slc30a3
Znt3

Purification Method

Affinity Purfied

UniProt Number

P97441

Molecular Weight

~45 kDa observed. 41.82 kDa (mouse) and 41.95 kDa (human) calculated.

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Alzheimer's diseased human brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected ZNT3 in 10 µg of Alzheimer's diseased human brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in Alzheimer's diseased human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ZNT3 in 10 µg of Alzheimer's diseased human brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-ZNT3 (rabbit polyclonal)

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