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Rb X TASK-2 Potassium Channel

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货号：AB5470-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5470-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Potassium Channel TASK-2 Antibody
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application Anti-Potassium Channel TASK-2 Antibody is an antibody against Potassium Channel TASK-2 for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1.5 μg/mL (1:200) on rat kidney membranes using ECL.

Immunohistochemistry on rat kidney tissue sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A purified peptide (TASK-2) corresponding to amino acid residues 483-499 of human TASK-2 (Accession O95279).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity The epitope is specific for TASK-2 and is not present in any other known proteins.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The message for this gene is mainly expressed in the cortical distal tubules and collecting ducts of the kidney. The protein is highly sensitive to external pH and this, in combination with its expression pattern, suggests it may play an important role in renal potassium transport.
Gene Symbol
  • KCNK5
  • FLJ11035
  • K2p5.1
  • TASK-2
  • TASK2
UniProt Number
UniProt Summary FUNCTION: SwissProt: O95279 # pH-dependent, voltage insensitive, outwardly rectifying potassium channel. Outward rectification is lost at high external K(+) concentrations.
SIZE: 499 amino acids; 55130 Da
SUBUNIT: Homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Abundant expression in kidney, also detected in liver, placenta and small intestine. In the kidney, expression is restricted to the distal tubules and collecting ducts. Not expressed in proximal tubules or glomeruli.
SIMILARITY: SwissProt: O95279 ## Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
MISCELLANEOUS: Inhibited by quinine, quinidine and external acidification.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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