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Rb X Ductin

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货号：AB5469

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Inv, Vrt ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5469
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Ductin Antibody
References
Product Information
Format Affinity Purified
Presentation Affinity purified. Liquid in 50% glycerol. Contains no preservative.
Applications
Application Anti-Ductin Antibody detects level of Ductin & has been published & validated for use in IC, IH & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Western Blot: 1:500-1:1,000.

Immunocytochemistry: 1:500

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide (100% conserved in all species) cooresponding to amino acids 115 to 126 of human ATP6VOC {gb AAH07389.1}.
Host Rabbit
Specificity Recognizes ductin (the 16 kDa or c' subunit of the V-ATPase).

SPECIES REACTIVITY:

The immunogen sequence is common to all species (plants to man). The antibody has specifically been tested on flatworm (invertebrate) and frog and chicken embryonic tissues (vertebrate) tissue.
Species Reactivity
  • Invertebrates
  • Vertebrates
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the V0 domain. This gene had the previous symbols of ATP6C and ATP6L.
Gene Symbol
  • ATP6V0C
  • VATL
  • Vma3
  • ATPL
  • ATP6C
  • ATP6L
  • EC 3.6.3.14
UniProt Number
UniProt Summary FUNCTION: SwissProt: P27449 # Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
SIZE: 155 amino acids; 15736 Da
SUBUNIT: V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein; which is present as a hexamer that forms the proton-conducting pore). Interacts with LASS2. Interacts with HTLV-1 accessory protein p12I.
SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P27449 ## Belongs to the V-ATPase proteolipid subunit family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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