Rb X P2X6 Receptor

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5477-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-P2X6 Receptor Antibody

Description

Catalogue Number

AB5477-200UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-P2X6 Receptor Antibody

Product Information
Format	Affinity Purified
Control	Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Product Information

Format

Affinity Purified

Control

Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Applications
Application	This Anti-P2X6 Receptor Antibody is validated for use in WB for the detection of P2X6 Receptor.
Key Applications	Western Blotting
Application Notes	Western blotting: 1:200 using ECL on rat brain membranes. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL 1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C. 2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours. 3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels) 4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 5. Destain with deionized water. 6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user. 8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted. 9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature. 10. Wash 4 x 10 min. with PBS-0.1% tween 20. 11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).

Applications

Application

This Anti-P2X6 Receptor Antibody is validated for use in WB for the detection of P2X6 Receptor.

Key Applications

Western Blotting

Application Notes

Western blotting: 1:200 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

SUGGESTED WESTERN BLOT PROTOCOL

1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

5. Destain with deionized water.

6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

10. Wash 4 x 10 min. with PBS-0.1% tween 20.

11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).

Biological Information
Immunogen	Highly purified peptide corresponding to residues 363-379 of rat P2X6 (Accession P51579).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins. SPECIES REACTIVITIES: The immunogen sequence is conserved in mouse (15/15) and human (14/17). Reactivity with other species has not yet been tested.
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_005446.3
Entrez Gene Summary	The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells.
Gene Symbol	P2RXL1 P2RX6 P2XM MGC129625 P2X6
UniProt Number	O15547
UniProt Summary	FUNCTION: SwissProt: O15547 # Receptor for ATP that acts as a ligand gated ion channel (By similarity). SIZE: 431 amino acids; 47913 Da SUBUNIT: Homo- or heteropolymers (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed predominantly in skeletal muscle. SIMILARITY: SwissProt: O15547 ## Belongs to the P2X receptor family.

Biological Information

Immunogen

Highly purified peptide corresponding to residues 363-379 of rat P2X6 (Accession P51579).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins.

SPECIES REACTIVITIES:

The immunogen sequence is conserved in mouse (15/15) and human (14/17). Reactivity with other species has not yet been tested.

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_005446.3

Entrez Gene Summary

The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells.

Gene Symbol

P2RXL1
P2RX6
P2XM
MGC129625
P2X6

UniProt Number

O15547

UniProt Summary

FUNCTION: SwissProt: O15547 # Receptor for ATP that acts as a ligand gated ion channel (By similarity).
SIZE: 431 amino acids; 47913 Da
SUBUNIT: Homo- or heteropolymers (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed predominantly in skeletal muscle.
SIMILARITY: SwissProt: O15547 ## Belongs to the P2X receptor family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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