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Rb X GlyRa3

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货号：AB5472-200UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R IHC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5472-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Glycine Receptor α3 Subunit Antibody
Alternate Names
  • GLRa3
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application Detect Glycine Receptor α3 Subunit using this Anti-Glycine Receptor α3 Subunit Antibody validated for use in IH.
Key Applications
  • Immunohistochemistry
Application Notes Western blot: 1:200 on rat brain membranes.

Immunohistochemistry on rat brain floating sections and mouse brain floating sections (trypsin pretreatment is recommended).

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A purified peptide corresponding to amino acid residues 355-368 of rat GlyRa3 (Accession P24524).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Glycine receptor alpha3 (GlyRalpha3) protein. The epitope is not similar to any other known proteins.
Species Reactivity
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLRA3
UniProt Number
UniProt Summary FUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
SIZE: 464 amino acids; 53800 Da
SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely distributed throughout the central nervous system.
DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
MISCELLANEOUS: The alpha subunit binds strychnine.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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