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Rb X Atrial Natriuretic Peptide

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货号：AB5490

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R IHC, IP, RIA Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5490
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Atrial Natriuretic Peptide Antibody
Alternate Names
  • ANP
References
Product Information
Format Affinity Purified
Control
  • Heart tissue
Presentation Affinity Purified immunoglobulin. Liquid containing 0.1% sodium azide.
Applications
Application This Anti-Atrial Natriuretic Peptide Antibody is validated for use in IH, IP, RIA for the detection of Atrial Natriuretic Peptide.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Radioimmunoassay
Application Notes Immunohistochemistry: 2-10 μg/mL. See suggested protocol below.

Immunoprecipitation

RIA

Optimal working dilutions must be determined by the end user.

IMMUNOHISTOCHEMISTRY PROTOCOL. Anesthetize animals and perfuse them transcardially as follows: a) Flush with cold (4°C) oxygenated Calcium free Tyrodes b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9 c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.ImmunofluorescenceDilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections for 24-48 hours at 2-8°C. Wash in PBS (three times for 10 minutes). Incubate for one hour or two hours at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin PeroxidaseIncubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining.
Biological Information
Immunogen Rat ANP conjugated to BSA.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Atrial Natriuretic Peptide (ANP).
Species Reactivity
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NPPA
  • CDD-ANF
  • ANP
  • PND
  • ANF
  • pronatriodilatin
  • OTTHUMP00000044485
  • Prepronatriodilatin
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01160 # A potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis. Has a cGMP-stimulating activity.
SIZE: 153 amino acids; 16708 Da
SUBUNIT: Interacts with NPR3.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01160 ## Belongs to the natriuretic peptide family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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