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GP X Prodynorphin Polyclonal Antibody

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0.00
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货号：AB5519

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC Gp Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5519
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Prodynorphin Antibody
References
Product Information
Format Affinity Purified
Presentation Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.
Applications
Application Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
Key Applications
  • Immunohistochemistry
Application Notes Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode's solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Biological Information
Immunogen Synthetic peptide from rat ProDynorphin.
Host Guinea Pig
Specificity ProDynorphin, rat.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine.
Gene Symbol
  • PDYN
  • MGC26418
  • preprodynorphin
  • leumorphin
  • beta-neoendorphin-dynorphin
  • rimorphin
  • prodynorphin
  • PENKB
  • Preprodynorphin
  • leu-enkephalin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01213 # Leumorphin has a typical opiod activity and may have anti-apoptotic effect (By similarity).
SIZE: 254 amino acids; 28385 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
SIMILARITY: SwissProt: P01213 ## Belongs to the opioid neuropeptide precursor family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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