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Mouse Anti-TIF1ß

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号:MAB3662

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, WB, ICC M Ascites Monoclonal Antibody
Description
Catalogue Number MAB3662
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-TIF1 beta Antibody
Alternate Names
  • TIF1beta
References
Product Information
Format Ascites
Presentation Ascites. Liquid. Contains no preservative.
Applications
Application Anti-TIF1 beta Antibody is a Mouse Monoclonal Antibody for detection of Transcriptional Intermediary Factor 1 also known as TIF1 beta & has been validated in ELISA, WB, ICC.
Key Applications
  • ELISA
  • Western Blotting
  • Immunocytochemistry
Application Notes Western blot: 1:500-1:5,000

Immunocytochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Recombinant protein from mouse TIF1b.
Host Mouse
Specificity Recognizes transcriptional intermediary factor 1beta (TIF1beta). The epitope is localized between amino acids 123-834 of mouse TIF1beta. No cross reactivity with TIF1alpha.
Isotype IgG2b
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Gene Symbol
  • TRIM28
  • FLJ29029
  • TIF1B
  • RNF96
  • TF1B
  • KAP-1
  • KRIP-1
  • TIF1-beta
  • KAP1
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q13263 # Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene (By similarity).
SIZE: 835 amino acids; 88550 Da
SUBUNIT: Associated with HP1 alpha (CBX5), beta (CBX1) and gamma (CBX3) in interphase nuclei (By similarity). Interacts with NCOR1 and CHD3. Interacts with SETDB1, ZFP53 and ZFP68. Interacts with CEBPB and NR3C1 (By similarity). Interacts directly with CBX5 via the PxVxL motif.
SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Associated with centromeric heterochromatin during cell differentiation through CBX1 (By similarity).
DOMAIN: SwissProt: Q13263 The HP1 box is both necessary and sufficient for HP1 binding. The RING finger domain and the B-box domains mediate interaction with CEBPB. The PHD domain enhances the CEBPB transcriptional activity (By similarity). & Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the TRIM/RBCC family. & Contains 2 B box-type zinc fingers. & Contains 1 bromo domain. & Contains 1 PHD-type zinc finger. & Contains 1 RING-type zinc finger.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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