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Ms X MMP-9

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货号：MAB13458

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB13458
Replaces 04-1150
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-MMP-9 Antibody, clone 9D4.2
Alternate Names
  • Gelatinase B
  • 92 kDa Type IV Collagenase
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in PBS containing 0.01% sodium azide.
Applications
Application Detect MMP-9 using this Anti-MMP-9 Antibody, clone 9D4.2 validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western blot: 1:200- 1:1000

Immunocytochemistry: Not Tested

ELISA: 1:200-1:1000

Immunohistochemistry: Not Tested

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Purified Human MMP-9 (Gelatinase B)
Clone 9D4.2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Human MMP-9 pro and active forms.
Isotype IgG2a
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Gene Symbol
  • MMP9
  • MMP-9
  • GELB
  • CLG4B
  • EC 3.4.24.35 [Contains: 67 kDa matrix metalloproteinase-9
  • 82 kDa matrix metalloproteinase-9].
UniProt Number
UniProt Summary FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
SIZE: 707 amino acids; 78427 Da
SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6/12 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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