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Anti-HCN2 (Rb Polyclonal)

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货号：AB5378-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5378-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-HCN2 Antibody
Alternate Names
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 2
  • BCNG2
  • HAC-1
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN:

    Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application This Anti-HCN2 Antibody is validated for use in IH, WB for the detection of HCN2.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:200-1:1,000 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified peptide from human HCN2 (amino acids 147-161) (Accession CAB42602).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes HCN2 protein. The epitope is specific for HCN2 and is not present in any other known protein.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HCN2
  • BCNG-2
  • HAC-1
  • BCNG2
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9UL51 # Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
SIZE: 889 amino acids; 96950 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed throughout the brain. Detected at low levels in heart.
DOMAIN:SwissProt: Q9UL51 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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