Anti-Sodium Channel NaV 1.7, Pain

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

IHC, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5390-50UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Sodium Channel Nav1.7 Antibody, Pain
Alternate Names	NENA NE SCN9A PN1

Description

Catalogue Number

AB5390-50UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Sodium Channel Nav1.7 Antibody, Pain

Alternate Names

NENA
NE
SCN9A
PN1

Product Information
Format	Affinity Purified
Control	CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Product Information

Format

Affinity Purified

Control

CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	Detect Sodium Channel Nav1.7 using this Anti-Sodium Channel Nav1.7 Antibody, Pain validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat dorsal root ganglion (DRG) frozen sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user.

Applications

Application

Detect Sodium Channel Nav1.7 using this Anti-Sodium Channel Nav1.7 Antibody, Pain validated for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat dorsal root ganglion (DRG) frozen sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Purified peptide from rat Nav1.7 (amino acids 446-460) (Accession AAB50403).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Nav1.7 protein (PN1, NENA, NE, Type IX Voltage Gated Sodium Channel, Scn9a). The epitope is specific for Nav1.7 and is not present in any other known protein. SPECIES REACTIVITIES: The immunogen sequence is highly conserved in rabbit and human (14/15 and 13/15 respectively). Other species have not been tested.
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002977.2
Gene Symbol	SCN9A NE-NA NENA ETHA Nav1.7 hNE-Na PN1
UniProt Number	Q15858
UniProt Summary	FUNCTION: SwissProt: Q15858 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (By similarity). SIZE: 1988 amino acids; 226342 Da SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4 and NEDD4L (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=In neurite terminals (By similarity). TISSUE SPECIFICITY: Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system while isoform 2 is expressed preferentially in the dorsal root ganglion. DOMAIN: SwissProt: Q15858 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. PTM: Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4 (By similarity). DISEASE: SwissProt: Q15858 # Defects in SCN9A are the cause of primary erythermalgia [MIM:133020]. It is an autosomal dominant disease characterized by recurrent episodes of severe pain associated with redness and warmth in the feet or hands. & Defects in SCN9A are the cause of autosomal recessive congenital indifference to pain [MIM:243000]; also known as channelopathy-associated insensitivity to pain. Affected individuals have a congenital inability to perceive any form of pain, in any part of the body. All other sensory modalities are preserved and the peripheral and central nervous systems are apparently intact. Patients perceive the sensations of touch, warm and cold temperature, proprioception, tickle and pressure, but not painful stimuli. There is no evidence of a motor or sensory neuropathy, either axonal or demyelinating. & Defects in SCN9A are a cause of paroxysmal extreme pain disorder (PEPD) [MIM:167400]; previously known as familial rectal pain (FRP). PEPD is an autosomal dominant paroxysmal disorder of pain and autonomic dysfunction. The distinctive features are paroxysmal episodes of burning pain in the rectal, ocular, and mandibular areas accompanied by autonomic manifestations such as skin flushing. SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.

Biological Information

Immunogen

Purified peptide from rat Nav1.7 (amino acids 446-460) (Accession AAB50403).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Nav1.7 protein (PN1, NENA, NE, Type IX Voltage Gated Sodium Channel, Scn9a). The epitope is specific for Nav1.7 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is highly conserved in rabbit and human (14/15 and 13/15 respectively). Other species have not been tested.

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_002977.2

Gene Symbol

SCN9A
NE-NA
NENA
ETHA
Nav1.7
hNE-Na
PN1

UniProt Number

Q15858

UniProt Summary

FUNCTION: SwissProt: Q15858 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (By similarity).
SIZE: 1988 amino acids; 226342 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4 and NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=In neurite terminals (By similarity).
TISSUE SPECIFICITY: Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system while isoform 2 is expressed preferentially in the dorsal root ganglion.
DOMAIN: SwissProt: Q15858 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4 (By similarity).
DISEASE: SwissProt: Q15858 # Defects in SCN9A are the cause of primary erythermalgia [MIM:133020]. It is an autosomal dominant disease characterized by recurrent episodes of severe pain associated with redness and warmth in the feet or hands. & Defects in SCN9A are the cause of autosomal recessive congenital indifference to pain [MIM:243000]; also known as channelopathy-associated insensitivity to pain. Affected individuals have a congenital inability to perceive any form of pain, in any part of the body. All other sensory modalities are preserved and the peripheral and central nervous systems are apparently intact. Patients perceive the sensations of touch, warm and cold temperature, proprioception, tickle and pressure, but not painful stimuli. There is no evidence of a motor or sensory neuropathy, either axonal or demyelinating. & Defects in SCN9A are a cause of paroxysmal extreme pain disorder (PEPD) [MIM:167400]; previously known as familial rectal pain (FRP). PEPD is an autosomal dominant paroxysmal disorder of pain and autonomic dysfunction. The distinctive features are paroxysmal episodes of burning pain in the rectal, ocular, and mandibular areas accompanied by autonomic manifestations such as skin flushing.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Sodium Channel NaV 1.7, Pain

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