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Anti-Opsin, Blue (Rabbit Polyclonal)

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货号：AB5407

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, Mk IH(P) Rb Purified Polyclonal Antibody
Description
Catalogue Number AB5407
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Opsin Antibody, blue
Background Information The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
References
Product Information
Format Purified
Control
  • Retina
Presentation Purified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative
Applications
Application This Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.
Key Applications
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Recombinant human blue opsin.
Epitope blue
Host Rabbit
Specificity Recognizes Opsin, blue.
Species Reactivity
  • Human
  • Mouse
  • Monkey
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • OPN1SW
  • CBT
  • BOP
  • BCP
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P03999 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
SIZE: 348 amino acids; 39135 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE:SwissProt: P03999 # Defects in OPN1SW are the cause of tritan color blindness (tritanopia) [MIM:190900].SIMILARITY:SwissProt: P03999 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at 2–8°C from date of shipment.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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