Rb X Ms Clock

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ELISA, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB5416P
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Clock Antibody
Alternate Names	KAT13D

Description

Catalogue Number

AB5416P

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Clock Antibody

Alternate Names

KAT13D

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin. Liquid in PBS and 0.1% BSA.

Product Information

Format

Affinity Purified

Presentation

Affinity purified immunoglobulin. Liquid in PBS and 0.1% BSA.

Applications
Application	Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.
Key Applications	ELISA Western Blotting
Application Notes	Western blot: 1-10 μg/mL using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.

Key Applications

ELISA
Western Blotting

Application Notes

Western blot: 1-10 μg/mL using ECL.

ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	An 18 amino acid peptide mapping downstream of the serine rich region of mouse Clock (Antoch et al. 1997; King et al. 1997).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes mouse Clock. The immunogen shows no significant sequence homology with dPER or other known proteins. SPECIES REACTIVITIES: The immunogen sequence is 92% conserved in rate and 90% in human. Reactivity with other species has not been confirmed.
Species Reactivity	Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004898.2
Entrez Gene Summary	This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
Gene Symbol	CLOCK KAT13D hCLOCK KIAA0334 clock
UniProt Number	O15516
UniProt Summary	FUNCTION: SwissProt: O15516 # BMAL1/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. SIZE: 846 amino acids; 95304 Da SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with BMAL1 is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and BMAL1. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-BMAL1 heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNT and ARNT2 to form heterodimers which bind poorly to the E-box motif (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus (By similarity). TISSUE SPECIFICITY: Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). PTM: Phosphorylation is dependent on CLOCK-BMAL1 heterodimer formation (By similarity). SIMILARITY: SwissProt: O15516 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains. MISCELLANEOUS: CLOCK-BMAL1 double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity.

Biological Information

Immunogen

An 18 amino acid peptide mapping downstream of the serine rich region of mouse Clock (Antoch et al. 1997; King et al. 1997).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes mouse Clock. The immunogen shows no significant sequence homology with dPER or other known proteins.

SPECIES REACTIVITIES: The immunogen sequence is 92% conserved in rate and 90% in human. Reactivity with other species has not been confirmed.

Species Reactivity

Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_004898.2

Entrez Gene Summary

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

Gene Symbol

CLOCK
KAT13D
hCLOCK
KIAA0334
clock

UniProt Number

O15516

UniProt Summary

FUNCTION: SwissProt: O15516 # BMAL1/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins.
SIZE: 846 amino acids; 95304 Da
SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with BMAL1 is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and BMAL1. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-BMAL1 heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNT and ARNT2 to form heterodimers which bind poorly to the E-box motif (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
PTM: Phosphorylation is dependent on CLOCK-BMAL1 heterodimer formation (By similarity).
SIMILARITY: SwissProt: O15516 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.
MISCELLANEOUS: CLOCK-BMAL1 double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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