Anti-Acetyl CoA Carboxylase 1, (Rabbit Monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1098
Replaces	04-322
Description	Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2
Alternate Names	acetyl-CoA carboxylase 1 acetyl-CoA carboxylase-alpha acetyl-Coenzyme A carboxylase alpha
Background Information	Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Description

Catalogue Number

05-1098

Replaces

04-322

Description

Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2

Alternate Names

acetyl-CoA carboxylase 1
acetyl-CoA carboxylase-alpha
acetyl-Coenzyme A carboxylase alpha

Background Information

Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Product Information
Format	Purified
Control	Human heart lysate
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Human heart lysate

Presentation

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate. Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.

Applications

Application

Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.

Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.

Biological Information
Immunogen	Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.
Clone	7D2.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG2aκ
Species Reactivity	Human Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_942131
Entrez Gene Summary	Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Gene Symbol	ACAC ACC ACC-alpha ACC1 ACCA
Purification Method	Protein G
UniProt Number	Q13085
UniProt Summary	FUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA. ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein. COFACTOR: Biotin. Binds 2 manganese ions per subunit. ENZYME REGULATION: By phosphorylation (By similarity). PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1. INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth. SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. Contains 1 carboxyltransferase domain.
Molecular Weight	~ 266 kDa Observed

Biological Information

Immunogen

Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.

Clone

7D2.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Isotype

IgG2aκ

Species Reactivity

Human
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_942131

Entrez Gene Summary

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Gene Symbol

ACAC
ACC
ACC-alpha
ACC1
ACCA

Purification Method

Protein G

UniProt Number

Q13085

UniProt Summary

FUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.

ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.

COFACTOR: Biotin.

Binds 2 manganese ions per subunit.

ENZYME REGULATION: By phosphorylation (By similarity).

PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.

INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.

SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Contains 1 carboxyltransferase domain.

Molecular Weight

~ 266 kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human heart lysate. Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human heart lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Acetyl CoA Carboxylase 1, (Rabbit Monoclonal)

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