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Anti-mTOR, rabbit monoclonal

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货号：04-385

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP M Purified Monoclonal Antibody
Description
Catalogue Number MABS196
Replaces 04-385
Description Anti-mTOR/FRAP Antibody, clone 22C2
Alternate Names
  • Serine/threonine-protein kinase mTOR
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • Mammalian target of rapamycin
  • mTOR
  • Mechanistic target of rapamycin
  • Rapamycin and FKBP12 target 1
  • Rapamycin target protein 1
Background Information The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.
References
Product Information
Format Purified
Control
  • Jurkat cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Detect the mTOR/FRAP protein using this Anti-mTOR Antibody, clone 22C2, validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Western Blot Analysis: A representative lot was used by an independent laboratory in neuroblastoma cells. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)
Biological Information
Immunogen Linear peptide corresponding to human mTOR/FRAP.
Clone 22C2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
Species Reactivity Note Demonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MTOR
  • FRAP
  • FRAP1
  • FRAP2
  • RAFT1
  • RAPT1
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~290 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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