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Qiagen 凯杰 Biosharp Omega

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Rb X MCT4

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号:AB3316P

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3316P
Replaces AB3548P
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Monocarboxylate Transporter 4 Antibody
Alternate Names
  • MCT4
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
Applications
Application Anti-Monocarboxylate Transporter 4 Antibody detects level of Monocarboxylate Transporter 4 & has been published & validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western blot: 1-5 μg/mL using ECL.
Immunohistochemistry: Not tested. Recommended antibody testing dilution: 5-10 μg/mL on paraformaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG219) per well.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A 19 amino acid peptide sequence within the cytoplasmic domain #4 of human MCT4 (Wilson et al., 1998; Price et al., 1998).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes human Monocarboxylate Transporter 4 (MCT4). The immunogen shows no significant sequence homology with other MCT. MCT4 is prominently expressed in skeletal muscle and other cells with high glycolytic rate such as tumors cells and white blood cells suggesting an important role in lactic acid efflux.

Note: According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Consult Halestrap and Price (1999) Biochem. J. 343:281-299.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM]
Gene Symbol
  • SLC16A3
  • MCT3
  • MGC138472
  • MGC138474
  • MCT4
UniProt Number
UniProt Summary FUNCTION: SwissProt: O15427 # Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).
SIZE: 465 amino acids; 49469 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle.
SIMILARITY: SwissProt: O15427 ## Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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