Rb X Endothelin Receptor B

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

IHC, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB3284-50UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Endothelin Receptor B Antibody
Alternate Names	ET-B

Description

Catalogue Number

AB3284-50UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Endothelin Receptor B Antibody

Alternate Names

ET-B

Product Information
Format	Affinity Purified
Control	Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Product Information

Format

Affinity Purified

Control

Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	Detect Endothelin Receptor B using this Anti-Endothelin Receptor B Antibody validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat brain sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user.

Applications

Application

Detect Endothelin Receptor B using this Anti-Endothelin Receptor B Antibody validated for use in IH & WB.

Key Applications

Immunohistochemistry
Western Blotting

Application Notes

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Purified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein. SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins Reactivity with other species has not been confirmed.
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000115.1 NM_003991.1
Entrez Gene Summary	The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct.
Gene Symbol	EDNRB ET-B ABCDS HSCR2 ETRB HSCR ETB
UniProt Number	P24530
UniProt Summary	FUNCTION: SwissProt: P24530 # Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. SIZE: 442 amino acids; 49644 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. PTM: Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. DISEASE: SwissProt: P24530 # Defects in EDNRB are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). & Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). It is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child. & Defects in EDNRB are the cause of ABCD syndrome [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. SIMILARITY: SwissProt: P24530 ## Belongs to the G-protein coupled receptor 1 family.

Biological Information

Immunogen

Purified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins Reactivity with other species has not been confirmed.

Species Reactivity

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct.

Gene Symbol

EDNRB
ET-B
ABCDS
HSCR2
ETRB
HSCR
ETB

UniProt Number

P24530

UniProt Summary

FUNCTION: SwissProt: P24530 # Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 442 amino acids; 49644 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
PTM: Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
DISEASE: SwissProt: P24530 # Defects in EDNRB are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). & Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). It is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child. & Defects in EDNRB are the cause of ABCD syndrome [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
SIMILARITY: SwissProt: P24530 ## Belongs to the G-protein coupled receptor 1 family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Rb X Endothelin Receptor B

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