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Ms X Human IgG Fc

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货号：MAB1302

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, RIA, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB1302
Replaces CBL102
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Mouse Anti-Human IgG Antibody, clone HP6017, Fc
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid in 0.02M PB, 0.25M NaCl with 0.1% sodium azide, pH=7.6.
Applications
Application Detect Human IgG using this Mouse anti-Human IgG Antibody, clone HP6017, Fc validated for use in ELISA, RIA & WB.
Key Applications
  • ELISA
  • Radioimmunoassay
  • Western Blotting
Application Notes Useful as both a capture antibody and a detection antibody for human IgG Fc (Papadea et al., 1985; Hamilton, et al., 1987).

ELISA

Radio-immunoassay (RIA)

Immunobloting.

Optimal working dilutions must be determined by end user.
Biological Information
Epitope Fc fragment
Clone HP6017
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Human IgG Fc fragment. ELISA Specificity: (Hamilton et al., 1987)

human IgG subclass 1 100%

human IgG subclass 2 100%

human IgG subclass 3 100%

human IgG subclass 4 100%

human IgG Pan 100%

human IgG Fc 100%

human IgG Fab .12%



Isoelectric Focusing: mean pI 8.0
Isotype IgG2aκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FCGR2B
  • CDw32
  • CD32B
  • IGFR2
  • CD32
  • Fc-gamma-RIIb
  • FCG2
  • FcRII-b
  • FCGR2
UniProt Number
UniProt Summary FUNCTION: SwissProt: P31994 # Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B- cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
SIZE: 310 amino acids; 34044 Da
SUBUNIT: Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Is the most broadly distributed Fc-gamma- receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
DOMAIN: SwissProt: P31994 Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2- containing phosphatases.DISEASE:SwissProt: P31994 # A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 500 µg
Transport Information
Supplemental Information
Specifications

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