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Anti-GODZ (rabbit polyclonal)

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货号：AB9556

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB9566
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Otx2 Antibody
References
Product Information
Format Serum
Presentation Rabbit serum. Liquid.
Applications
Application Anti-Otx2 Antibody detects level of Otx2 & has been published & validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Western blot: 1:500-1:4,000. The antibody reacts with a doublet at 36 kDa in adult mouse retina extract.

Immunohistochemistry: 1:500-1:4,000. Suggested fixative is 4% PFA-perfused or immersion fixed tissue. Suggested permeablization method is Triton X100 and blocking buffer is 5% serum in PBS. Suggested incubation time is overnight at 2-8°C.

Immunocytochemistry

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Full length recombinant human OTX2 (excluding the first 5 amino acids).
Host Rabbit
Specificity The antibody recognizes OTX2. The antibody will also react with OTX1 due to amino acid sequence homology between the two proteins. OTX2 is expressed earlier than OTX1 and therefore the antibody can be used to identify OTX2 during development.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined.
Gene Symbol
  • OTX2
  • MCOPS5
  • MGC45000
UniProt Number
UniProt Summary FUNCTION: SwissProt: P32243 # Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
SIZE: 289 amino acids; 31636 Da
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Expressed in brain.
DEVELOPMENTAL STAGE: Embryo.
DISEASE: SwissProt: P32243 # Defects in OTX2 are the cause of syndromic microphthalmia 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities such as cardiac defects, facial clefts, microcephaly and hydrocephaly. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
SIMILARITY: SwissProt: P32243 ## Belongs to the paired homeobox family. Bicoid subfamily. & Contains 1 homeobox DNA-binding domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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