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Rb X EphrinB, phosphoTyrosine298

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货号：AB9624

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB9624
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Ephrin B phosphoTyr298 Antibody

References

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin. Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Applications
Application	Anti-Ephrin B phosphoTyr298 Antibody detects level of Ephrin B phosphoTyr298 & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western blot: 1:1,000. This antibody detects a strong band around 46kDa in rat testes lysate. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide of amino acids surrounding the phosphoTyrosine 298 site of Xenopus EphrinB. Xenopus Tyrosine 298 is the homolog of mouse, rat and human Tyrosine 317 and chicken Tyrosine 305.
Host	Rabbit
Specificity	EphrinB, phosphoTyrosine 298. The antibody recognizes a protein of ~46 kDa corresponding to EphrinB, phosphoTyrosine 298 in lysates from rat testes. Immunolabeling is blocked by l-phosphatase treatment.
Species Reactivity	Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004441.3
Entrez Gene Summary	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Gene Symbol	EPHB1 NET Elk FLJ37986 Hek6 ELK EPHT2 EphB1 HEK6 EC 2.7.10.1
Modifications	Phosphorylation
UniProt Number	P54762
UniProt Summary	FUNCTION: SwissProt: P54762 # Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system. SIZE: 984 amino acids; 109885 Da SUBUNIT: The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Preferentially expressed in brain. SIMILARITY: SwissProt: P54762 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. & Contains 1 SAM (sterile alpha motif) domain.

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Packaging Information
Material Size	100 µL

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