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Rb X PSD-95 Affinity Purified

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货号：AB9634

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB9634
Replaces 04-1066
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Post Synaptic Density 95 Antibody
Alternate Names
  • PSD-95
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Applications
Application This Anti-Post Synaptic Density 95 Antibody is validated for use in WB for the detection of Post Synaptic Density 95.
Key Applications
  • Western Blotting
Application Notes Western blot: 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide from the N-terminal of rat PSD-95.
Host Rabbit
Specificity Postsynaptic density 95 (PSD-95). The antibody recognizes a protein of ~95 kDa corresponding to PSD-95 in lysates from rat brain hippocampus. An additional band of ~44 kDa may also be seen depending on sample and antibody concentration used.
Isotype IgG
Species Reactivity
  • Rat
Species Reactivity Note The immunogen has 100% similarity with human and mouse.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins.
Gene Symbol
  • DLG4
  • SAP90
  • PSD-95
  • PSD95
UniProt Number
UniProt Summary FUNCTION: SwissProt: P78352 # Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity).
SIZE: 724 amino acids; 80495 Da
SUBUNIT: Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, and BEGAIN (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
TISSUE SPECIFICITY: Brain.
DOMAIN: SwissProt: P78352 The PDZ domain 3 mediates interaction with ADR1B. & The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
PTM: Palmitoylation of isoform 1 is required for targeting to postsynaptic density (By similarity).
SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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