RABBIT ANTI-CHAPSYN-110 PURIFIED POLYCLONAL ANTIBODY - 炼石商城

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RABBIT ANTI-CHAPSYN-110 PURIFIED POLYCLONAL ANTIBODY

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货号：AB5168-200UL

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB, IHC	Rb	Purified	Polyclonal Antibody

Description
Catalogue Number	AB5168-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-CHAPSYN-110 Antibody
Alternate Names	PSD-93 Discs Large Homolog 2 DLG2

References

Product Information
Format	Purified
Control	CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 10 ng of protein per lane. For negative control, preincubate 1 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	This Anti-CHAPSYN-110 Antibody is validated for use in WB, IH for the detection of CHAPSYN-110.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Western blot: 1 to 3 μg/mL (1:300-1:1000) using ECL Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	GST fusion protein (Smith & Johnson 1988) and of rat chapsyn-110 (amino acids 336-379) (Brenman et al. 1996; Kim et al. 1996).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes a full length chapsyn-110 protein. It has exhibited no cross reactivity with other known related proteins tested so far.
Species Reactivity	Rat
Species Reactivity Note	The immunogen sequence is 42 of 44 amino acids identical in human.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001364.2
Entrez Gene Summary	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Alternatively spliced transcript variants encoding distinct isoforms have been described but their full-length nature has yet to be completely determined.
Gene Symbol	DLG2 PSD-93 DKFZp781E0954 Chapsyn-110 FLJ37266 MGC131811 chapsyn-110 DKFZp781D1854
UniProt Number	Q15700
UniProt Summary	FUNCTION: SwissProt: Q15700 # Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity). SIZE: 870 amino acids; 97542 Da SUBUNIT: Interacts with NOS1/nNOS through second PDZ domain (By similarity). Interacts with C-terminus of KCNJ2/Kir2.1 through one of its PDZ domains. SUBCELLULAR LOCATION: Membrane; Lipid-anchor (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Concentrated in soma and postsynaptic density of a subset of neurons (By similarity). DOMAIN:SwissProt: Q15700 Isoform 2 has an L27 domain close to N-terminus. PTM: Palmitoylation of isoform 1 is not required for targeting to postsynaptic density (By similarity). SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain.

Physicochemical Information

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Materials Information

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Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	200 µL

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