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Rb X P2X4 Purinergic Receptor

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货号：AB5226-50UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB5226-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-P2X4 Receptor Antibody
References
Product Information
Format Affinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application Anti-P2X4 Receptor Antibody detects level of P2X4 Receptor & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application Notes Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen A purified peptide of rat P2X4 protein (amino acids 370-388) (Accession P51577).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes a full-length P2X4 purinergic receptor protein and not with other known proteins tested so far.

SPECIES REACTIVITIES: The immunogen sequence is identical in mouse. Other species have not been tested.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
Gene Symbol
  • P2RX4
  • P2X4R
  • P2X4
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q99571 # Receptor for ATP that acts as a ligand gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
SIZE: 388 amino acids; 43369 Da
SUBUNIT: Homo- or heteropolymers (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q99571 ## Belongs to the P2X receptor family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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