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Anti-A2B5 (Mouse Monoclonal)

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货号：MAB312R

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ma FC, ICC, IF, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB312R
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-A2B5 Antibody, clone A2B5-105
Alternate Names
  • Neuron Cell Surface Antigen
Background Information A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.
References
Product Information
Format Purified
Control
  • Type II astrocytes, human neural progenitors
Presentation Liquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA.
Applications
Application Anti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
Application Notes Immunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989)

Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Embryonic chicken retinal cells
Clone A2B5-105
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:

Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer's disease (Majocha et al., 1989)
Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
Human and rat thymic epithelial cells (Haynes et al., 1988).
Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
Isotype IgM
Species Reactivity
  • Mammals
Antibody Type Monoclonal Antibody
Gene Symbol
  • A2B5
Purification Method Ammonium Sulfate Precipitation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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