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Ms Anti-Surfactant Protein D (SP-D) Lectin Domain

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货号：MAB3132

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, R ELISA, IH(P), WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB3132
Replaces MAB3130
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11
Alternate Names
  • SP-D
Background Information Surfactant protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620 kDa under non-reducing conditions and 43 kDa under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A.
References
Product Information
Format Purified
Presentation Immunoglobulin purified from tissue culture supernatant. Lyophilized from buffer containing 5 mg/mL BSA and 0.09% sodium azide.
Applications
Application This Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11 is validated for use in ELISA, IH(P), WB for the detection of Surfactant Protein D.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunohistochemistry on snap frozen or paraffin embedded tissue.

Suggested working dilution for fresh rat tissue is 1:400 and for fresh human tissue is 1:20. Fixation: acetone 5 min at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W for paraffin tissues.

Western blot

ELISA: as capture antibody

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified rat surfactant protein D from bronchoalveolar lavage.
Epitope lectin domain
Clone IVF11
Host Mouse
Specificity MAB3132 recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells (in which SpD is highly concentrated), SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident at very low dilutions (1 to less than a few hundred) by ELISA.
Isotype IgG1κ
Species Reactivity
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SFTPD
  • SP-D
  • SFTP4
  • PSPD
  • PSP-D
  • COLEC7
UniProt Number
UniProt Summary FUNCTION: SwissProt: P35247 # Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
SIZE: 375 amino acids; 37698 Da
SUBUNIT: Oligomeric complex of 4 set of homotrimers.
SUBCELLULAR LOCATION: Secreted, extracellular space.
PTM: The N-terminus is blocked. & Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates (By similarity).
SIMILARITY: SwissProt: P35247 ## Contains 1 C-type lectin domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at 2-8°C for up to 12 months from date of receipt. Reconstitute lyophilized material with 500 μL of sterile distilled water. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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