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Ms X NSE

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货号：IHCR2014-6

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number IHCR2014-6
Trade Name
  • IHC Select
  • Chemicon
Description IHC Select® Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14
Alternate Names
  • NSE
References
Product Information
Format Purified
Presentation Monoclonal mouse antibody supplied in liquid format as tissue culture supernatant, diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as a preservative.
Applications
Application This IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14 is validated for use in IH(P) for the detection of Neuron-Specific Enolase.
Key Applications
  • Immunohistochemistry (Paraffin)
Application Notes Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select® Detection Kits.

NSE has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2014-6.
Biological Information
Immunogen Gamma-gamma enolase purified from human brain
Clone BBS/NC/VI-H14
Host Mouse
Specificity Reacts with human gamma-gamma enolase (Mr 46000). This antibody does not cross react with alpha or beta subunits of NSE.
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Gene Symbol
  • ENO2
  • NSE
  • Gamma-enolase
  • EC 4.2.1.11
UniProt Number
UniProt Summary FUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
SIZE: 434 amino acids; 47269 Da
SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C . Refer to vial for expiration dating.
Packaging Information
Material Size 6 mL
Transport Information
Supplemental Information
Specifications

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