Ms Anti-Hu CD93 (C1qRp) Alexa Fluor® 488 Conjugate

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Ms Anti-Hu CD93 (C1qRp) Alexa Fluor® 488 Conjugate

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货号：MAB4314X

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB4314
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-CD93 Antibody, extracellular, clone R139
Alternate Names	Complement C1q Receptor

References

Product Information
Format	Purified
Presentation	Purified immunoglobulin. Liquid in 0.02 M Phosphate with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.

Applications
Application	This Anti-CD93 Antibody, extracellular, clone R139 is validated for use in FC, IP, WB for the detection of CD93.
Key Applications	Flow Cytometry Immunoprecipitation Western Blotting
Application Notes	Immunocytochemistry: Recommended antibody dilution: 5-10 μg/ml Fixative: 4% Paraformaldehyde / PBS Blocking buffer: 2% BSA / 2% Normal serum / PBS Dilution Buffer: 2% BSA / 2% Normal serum / PBS Incubation Times/Temperature: 1 hour at room temperature Recommended control tissue: Positive: U937 monocytes; Negative: T cells Immunoblotting: Recommended primary antibody dilution: 5 μg/ml Dilution buffer: 3% milk powder / 0.05%Tween 20 in Tris buffered saline Sample preparation: Denatured only (SDS & boiled) Preferred Gel percentage: 7.5% Polyacrylamide Blocking Buffer: 3% milk powder / 0.05% Tween 20 in Tris buffered saline Incubation time: 2 hours at room temperature Expected size of band: 97 kDa Recommended control extracts: Positive: U937 monocytes; Negative: T cells Immunoprecipitation: Recommended primary antibody dilution: 5 μg/ml Cell lysis buffer: 10mM TEA, 1mM Calcium Cholride, 0.15M Sodium Chloride, 0.3% NP40 and protease inhibitors Final Reaction volume: 1000 μl Suggested total protein concentration: 0.15 mg/ml (1 x 107 cells) Incubation time: 1 hour at 4°C Expected size of band: 97kDa ELISA (Indirect): Recommended coating concentration: 10 μg/ml Dilution buffer: 0.1M Sodium Carbonate pH9.4 FACS Analysis: Suggested dilution/number of cells: 2 μg/million cells Fixation/Permeabilization used: 1% Paraformaldehyde / PBS Recommended controls: U937 Monocytes Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	C1q collagen like domain binding proteins
Epitope	extracellular
Clone	R139
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes human CD93 (C1qRp), a 90-120kD protein that is highly expressed on monocytes/macrophages, neutrophils and granulocytes. CD93 is not expressed upon T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). However, CD93 is not necessary for the binding of C1q or for inducing C1q-mediated enhancement of phagocytic activity.Recent evidence suggests that CD93 may define a new human stem cell population with hematopoietic and hepatic potential3.
Isotype	IgG2b
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_012072.3
Entrez Gene Summary	The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.
Gene Symbol	CD93 MXRA4 C1qRp C1qR(P) C1qR dJ737E23.1 C1QR1 CDw93 p C1qRP
UniProt Number	Q9NPY3
UniProt Summary	FUNCTION: SwissProt: Q9NPY3 # Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. SIZE: 652 amino acids; 68560 Da SUBUNIT: Interacts with HCV core protein. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. PTM: N- and O-glycosylated. SIMILARITY: SwissProt: Q9NPY3 ## Contains 1 C-type lectin domain. & Contains 5 EGF-like domains.
Stem Cell Type	Hematopoietic Stem Cells

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Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Packaging Information
Material Size	100 µg

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Ms Anti-Hu CD93 (C1qRp) Alexa Fluor® 488 Conjugate

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