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Ms X Kinesin (Heavy Chain)

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货号：MAB1613

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H, R ELISA, IP, RIA, WB, ICC, IHC, FUNC M Purified Monoclonal Antibody
Description
Catalogue Number MAB1613
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1
References
Product Information
Format Purified
Presentation Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl.pH 7.6 with 0.1% sodium azide.
Applications
Application Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1 detects level of Kinesin & has been published & validated for use in ELISA, IP, RIA, WB, IC, IH.
Key Applications
  • ELISA
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Affects Function
Application Notes Immunohistochemistry on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry: paraformaldehyde fixed cells; 5-10' RT, freshly made 4% PFA; permeabilize with 0.1-.2% triton X-100 in 3% normal serum blocking buffer

Immunoblotting: recognizes 124kDa KHC from non-neuronal samples.

Immunoprecipitation

Inhibition of kinesin mediated transport

EIA/RIA

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Bovine brain kinesin.
Epitope heavy chain, non-neuronal KHC
Clone H1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Reacts with the major form of heavy chain of non-neuronal KHC near the N-terminus.
Isotype IgG1
Species Reactivity
  • Bovine
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KIF5B
  • UKHC
  • KINH
  • KNS1
  • KNS
UniProt Number
UniProt Summary FUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
SIZE: 963 amino acids; 109685 Da
SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU.
SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity).
DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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