Rabbit Anti-BCL-2

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R	WB, IP, IHC	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R

WB, IP, IHC

Serum

Polyclonal Antibody

Description
Catalogue Number	AB1722
Replaces	04-436
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2
Background Information	In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax). {http://www.celldeath.de/encyclo/}

Description

Catalogue Number

AB1722

Replaces

04-436

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2

Background Information

In C. elegans, ced-3 and ced-4 were identified as genes essential for programmed cell death, while ced-9 was found to be a regulator of cell death by preventing apoptosis. The first mammalian homolog for ced-3 was described in 1988 as Bcl-2 which is involved in B-cell lymphomas (Vaux and Adams, 1988, Nature 335: 440). Bcl-2 transfected B cells were shown to be resistant towards apoptosis normally induced in B cells by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis. The bcl-2 gene codes for a 25 kDa protein. The C terminal 21 amino acids encode a stretch of hydrophobic amino acids that are important in membrane docking: Bcl-2 resides on the cytoplasmic face of the mitochondrial outer membrane, the nuclear envelop, and the endoplasmic reticulum. Deletion of the C terminus does not abrogate Bcl-2 survival function! Most Bcl-2 homologs have this hydrophobic C terminal domain, though they not necessarily are located on membranes but are cytosolic (e.g. Bax).

{http://www.celldeath.de/encyclo/}

Product Information
Format	Serum
Presentation	Whole serum without preservatives

Product Information

Format

Serum

Presentation

Whole serum without preservatives

Applications
Application	Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 is an antibody against Bcl-2 for use in WB, IP, IH.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry
Application Notes	Immunoblotting: Recommended antibody dilution 1:1500-3000 Immunoprecipitation: Recommended antibody dilution 1:50 Immunohistochemistry: Recommended antibody dilution 1:600-1500 Optimal antibody dilutions must be determined by end-user.

Applications

Application

Anti-Bcl-2 Antibody, a.a. 68-86 of mBCL2 is an antibody against Bcl-2 for use in WB, IP, IH.

Key Applications

Western Blotting
Immunoprecipitation
Immunohistochemistry

Application Notes

Immunoblotting: Recommended antibody dilution 1:1500-3000

Immunoprecipitation: Recommended antibody dilution 1:50

Immunohistochemistry: Recommended antibody dilution 1:600-1500

Optimal antibody dilutions must be determined by end-user.

Biological Information
Immunogen	Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV
Epitope	a.a. 68-86 of mBCL2
Host	Rabbit
Specificity	AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown
Species Reactivity	Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000633.2 NM_000657.2
Entrez Gene Summary	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Gene Symbol	BCL2 Bcl-2
UniProt Number	P10415
UniProt Summary	FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). SIZE: 239 amino acids; 26266 Da SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1. PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. SIMILARITY: Belongs to the Bcl-2 family.

Biological Information

Immunogen

Peptide against mouse Bcl-2 a.a. 68-86 RTSPLRPLVATAGPALSPV

Epitope

a.a. 68-86 of mBCL2

Host

Rabbit

Specificity

AB1722 recognizes Bcl-2 alpha and beta isoforms in mouse and rat. Cross reactivity with other species is unknown

Species Reactivity

Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Gene Symbol

BCL2
Bcl-2

UniProt Number

P10415

UniProt Summary

FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
SIMILARITY: Belongs to the Bcl-2 family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws.

Storage and Shipping Information

Storage Conditions

Store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze thaws.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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