beta 1 INTEGRIN INVESTIGATOR KIT

Description
Catalogue Number	ECM455
Brand Family	Chemicon®
Trade Name	Chemicon
Description	β1 Integrin Polyclonal Antibody Kit
Overview	CHEMICON Investigator Kits for Integrin Research are a cost-effective source of reagents for all investigators intimately involved with this expanding field of study. The beta1 Integrin Polyclonal Investigator Kit is designed for research focused on the human beta1 integrin subunit and those integrin subunits known to pair with beta1, including alpha1, alpha2, alpha3, alpha4, alpha5 and alphaV. Integrins belong to a large family of cell surface proteins commonly referred to as glycoproteins. Integrins are composed of noncovalently associated alpha and beta subunits which make up heterodimers: the larger termed alpha chain and the smaller beta chain. At least 16 alpha subunits and 8 beta subunits are expressed, which can give rise to 22 different integrins. Integrins are thought to play critical roles in cell migration, differentiation, and survival. There is now considerable evidence that these transmembrane receptors play an important role in the regulation of gene expression and signal transduction. Excellent reviews of integrin structure, function, and ligand specificity may be found in: Hynes, R. 1992, Cell 69:11-25; Kramer, R. 1993. "Integrin Structure and Ligand Specificity in Cell-Matrix Interactions" in Molecular and Cellular Aspects of Basement Membranes. Acedemic Press. Chemicon offers the beta1 Integrin Polyclonal Investigator Kit for initial studies of beta1 subunit interactions. All kit reagents are also available in larger sizes. The reagents in this kit are not application specific but rather may be used in a variety of procedures, depending upon the needs of the individual investigtor.

Description

Catalogue Number

ECM455

Brand Family

Chemicon®

Trade Name

Chemicon

Description

β1 Integrin Polyclonal Antibody Kit

Overview

CHEMICON Investigator Kits for Integrin Research are a cost-effective source of reagents for all investigators intimately involved with this expanding field of study. The beta1 Integrin Polyclonal Investigator Kit is designed for research focused on the human beta1 integrin subunit and those integrin subunits known to pair with beta1, including alpha1, alpha2, alpha3, alpha4, alpha5 and alphaV.

Integrins belong to a large family of cell surface proteins commonly referred to as glycoproteins. Integrins are composed of noncovalently associated alpha and beta subunits which make up heterodimers: the larger termed alpha chain and the smaller beta chain. At least 16 alpha subunits and 8 beta subunits are expressed, which can give rise to 22 different integrins. Integrins are thought to play critical roles in cell migration, differentiation, and survival. There is now considerable evidence that these transmembrane receptors play an important role in the regulation of gene expression and signal transduction. Excellent reviews of integrin structure, function, and ligand specificity may be found in: Hynes, R. 1992, Cell 69:11-25; Kramer, R. 1993. "Integrin Structure and Ligand Specificity in Cell-Matrix Interactions" in Molecular and Cellular Aspects of Basement Membranes. Acedemic Press.

Chemicon offers the beta1 Integrin Polyclonal Investigator Kit for initial studies of beta1 subunit interactions. All kit reagents are also available in larger sizes. The reagents in this kit are not application specific but rather may be used in a variety of procedures, depending upon the needs of the individual investigtor.

Product Information
Components	beta1 Polyclonal Antibody (Part No. AB1952-20)- 1 tube, 20 μL. alpha1 Polyclonal Antibody (Part No. AB1934-20) - 1 tube, 20 μL. alpha2 Polyclonal Antibody (Part No. AB1944-20)- 1 tube, 20 μL. alpha3 Polyclonal Antibody (Part No. AB1920-20) - 1 tube, 20 μL. alpha4 Polyclonal Antibody (Part No. AB1924-20) - 1 tube, 20 μL. alpha5 Polyclonal Antibody (Part No. AB1949-20) - 1 tube, 20 μL. alphaV Polyclonal Antibody (Part No. AB1930-20)- 1 tube, 20 μL.
Presentation	With 20 μL each of polyclonals to beta1, alpha1, alpha2, alpha3, alpha4, alpha5 and alphaV.

Product Information

Components

beta1 Polyclonal Antibody (Part No. AB1952-20)- 1 tube, 20 μL.
alpha1 Polyclonal Antibody (Part No. AB1934-20) - 1 tube, 20 μL.
alpha2 Polyclonal Antibody (Part No. AB1944-20)- 1 tube, 20 μL.
alpha3 Polyclonal Antibody (Part No. AB1920-20) - 1 tube, 20 μL.
alpha4 Polyclonal Antibody (Part No. AB1924-20) - 1 tube, 20 μL.
alpha5 Polyclonal Antibody (Part No. AB1949-20) - 1 tube, 20 μL.
alphaV Polyclonal Antibody (Part No. AB1930-20)- 1 tube, 20 μL.

Presentation

With 20 μL each of polyclonals to beta1, alpha1, alpha2, alpha3, alpha4, alpha5 and alphaV.

Applications
Application	This β1 Integrin polyclonal antibody Kit is validated for use in ELISA, IHC, western blotting & IP for the detection of β1 Integrin Polyclonal Kit.
Key Applications	ELISA Immunohistochemistry Western Blotting Immunoprecipitation

Applications

Application

This β1 Integrin polyclonal antibody Kit is validated for use in ELISA, IHC, western blotting & IP for the detection of β1 Integrin Polyclonal Kit.

Key Applications

ELISA
Immunohistochemistry
Western Blotting
Immunoprecipitation

Biological Information
Species Reactivity	Human
Entrez Gene Number	NM_002211.2 NM_033666.1 NM_033667.1 NM_033668.1 NM_033669.1 NM_133376.1
Entrez Gene Summary	Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Gene Symbol	ITGB1 GPIIA VLAB OTTHUMP00000063732 MDF2 FNRB OTTHUMP00000063731 OTTHUMP00000046253 CD29 OTTHUMP00000063733 MSK12
UniProt Number	P05556
UniProt Summary	FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. SIZE: 798 amino acids; 88465 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle). SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.

Biological Information

Species Reactivity

Human

Entrez Gene Number

Entrez Gene Summary

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Gene Symbol

ITGB1
GPIIA
VLAB
OTTHUMP00000063732
MDF2
FNRB
OTTHUMP00000063731
OTTHUMP00000046253
CD29
OTTHUMP00000063733
MSK12

UniProt Number

P05556

UniProt Summary

FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

SIZE: 798 amino acids; 88465 Da

SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).

SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store antibodies at -20°C. Important Note: Due to the small volumes contained within the kit, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge before opening to dislodge any liquid entrapped in the container's cap during shipment.

Storage and Shipping Information

Storage Conditions

Store antibodies at -20°C.

Important Note: Due to the small volumes contained within the kit, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge before opening to dislodge any liquid entrapped in the container's cap during shipment.

Packaging Information
Material Size	1 kit

Packaging Information

Material Size

1 kit

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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beta 1 INTEGRIN INVESTIGATOR KIT

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