PAI Activity Assay Kit

Species Reactivity	Key Applications
H	ACT

Species Reactivity

Key Applications

ACT

Description
Catalogue Number	ECM610
Brand Family	Chemicon®
Trade Name	Chemicon
Description	PAI Activity Assay
Overview	The CHEMICON PAI Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of inhibition of uPA activity by PAI and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over an inhibition range of 0.05-50 units of uPA activity. The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA. The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA. Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with > 45% of active form. Duplicate or triplicate samples are suggested. The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications. Test Principle: The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA. Application: The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA. Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with > 45% of active form. Duplicate or triplicate samples are suggested. The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Materials Required but Not Delivered	1. Single or Multichannel Pipette and disposable tips 2. Microplate reader (405 nm) 3. 37°C incubator 4. Clean 96-well microplate for performing incubations. 5. Activation Buffers

Description

Catalogue Number

ECM610

Brand Family

Chemicon®

Trade Name

Chemicon

Description

PAI Activity Assay

Overview

The CHEMICON PAI Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of inhibition of uPA activity by PAI and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over an inhibition range of 0.05-50 units of uPA activity.

The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.

The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.

Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with > 45% of active form. Duplicate or triplicate samples are suggested.

The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Test Principle:

The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.

Application:

The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.

Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with > 45% of active form. Duplicate or triplicate samples are suggested.

The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Materials Required but Not Delivered

1. Single or Multichannel Pipette and disposable tips

2. Microplate reader (405 nm)

3. 37°C incubator

4. Clean 96-well microplate for performing incubations.

5. Activation Buffers

Product Information
Components	uPA Standard: (Part No. 90092) One lyophilized vial, 1000 units, of uPA from human urine. Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group. Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle. PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.

Product Information

Components

uPA Standard: (Part No. 90092) One lyophilized vial, 1000 units, of uPA from human urine.
Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group.
Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle.
PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.

Applications
Application	The PAI Activity Assay Kit provides a quick, efficient & sensitive system for evaluation of inhibition of uPA activity by PAI & for screening of uPA inhibitors.
Key Applications	Activity Assay

Applications

Application

The PAI Activity Assay Kit provides a quick, efficient & sensitive system for evaluation of inhibition of uPA activity by PAI & for screening of uPA inhibitors.

Key Applications

Activity Assay

Biological Information
Species Reactivity	Human
Entrez Gene Number	NM_002658.2
Entrez Gene Summary	This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
Gene Symbol	PLAU UPA URK u-PA uPA ATF EC 3.4.21.73 [Contains: Urokinase-type plasminogen activator long chain A Urokinase-type plasminogen activator short chain A Urokinase-type plasminogen activator chain B].
UniProt Number	P00749
UniProt Summary	FUNCTION: SwissProt: P00749 # Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. SIZE: 431 amino acids; 48525 Da SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in the prostate gland and prostate cancers. PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. SIMILARITY: SwissProt: P00749 ## Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 kringle domain. & Contains 1 peptidase S1 domain.

Biological Information

Species Reactivity

Human

Entrez Gene Number

NM_002658.2

Entrez Gene Summary

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.

Gene Symbol

PLAU
UPA
URK
u-PA
uPA
ATF
EC 3.4.21.73 [Contains: Urokinase-type plasminogen activator long chain A
Urokinase-type plasminogen activator short chain A
Urokinase-type plasminogen activator chain B].

UniProt Number

P00749

UniProt Summary

FUNCTION: SwissProt: P00749 # Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
SIZE: 431 amino acids; 48525 Da
SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in the prostate gland and prostate cancers.
PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
SIMILARITY: SwissProt: P00749 ## Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 kringle domain. & Contains 1 peptidase S1 domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store kit materials at -20°C for up to 12 months. Store reconstituted chromogenic substrate at 2-8°C.

Storage and Shipping Information

Storage Conditions

Store kit materials at -20°C for up to 12 months. Store reconstituted chromogenic substrate at 2-8°C.

Packaging Information
Material Size	1 plate
Material Package	96 wells

Packaging Information

Material Size

1 plate

Material Package

96 wells

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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